In regenerative medical merchandise for medical purposes, a significant concern is the danger of ruminant-derived supplies creating transmissible spongiform encephalopathy (TSE) within the manufacturing course of. Due to the danger of TSE inflicting prion illness, the uncooked supplies derived from ruminants ought to be compliant with the “Customary for Organic Uncooked Supplies” to make sure the standard and security of pharmaceutical merchandise.
We due to this fact examined whether or not plasmid DNA may face up to 4 chemical reagents (Gdn-HCl, Gdn-SCN, TCA, or SDS), having referred to the report by Tateishi et al. [1], which describes how Creutzfeldt-Jakob illness pathogens will be inactivated by chemical reagents able to producing a 7-log discount in prion inactivation. We noticed that plasmid DNA was combined with chemical reagents and that the performance of plasmid DNA was equal for each chemical and non-chemical remedy.
The efficiency of plasmid DNA was monitored by the existence of DNA fragments and the perform by which GFP proteins had been produced by HEK293-cell transfected plasmid DNA. The existence of DNA fragments was detected in plasmid DNA handled by chemical reagents, besides when present process TCA remedy. Moreover, when HEK293 cells had been transfected with the plasmid DNA after chemical remedy, GFP protein was produced. These outcomes point out that plasmid DNA can face up to the chemical therapies for blocking prion transmission.
Identification of a Potential Membrane-Concentrating on Sequence within the C-Terminus of the F Plasmid Segregation Protein SopA
Steady upkeep and partitioning of the ‘Fertility’ plasmid or the F plasmid in its host Escherichia coli require the perform of a ParA superfamily of proteins often called SopA. The mechanism by which SopA mediates plasmid segregation is effectively studied. SopA is a nucleoid-binding protein and binds DNA in an ATP-dependent however sequence non-specific method. ATP hydrolysis stimulated by the binding of the SopBC complicated mediates the discharge of SopA from the nucleoid.
Cycles of ATP-binding and hydrolysis generate an ATPase gradient that strikes the plasmid by a chemophoresis pressure. Nucleoid binding of SopA thus assumes a central function in its plasmid-partitioning perform. Nevertheless, earlier work additionally means that the F plasmid will be partitioned into anucleate cells, thus implicating nucleoid impartial partitioning. Curiously, SopA can be reported to be related to the inside membrane of the micro organism. Right here, we report the identification of a doable membrane-targeting sequence, a predicted amphipathic helix, on the C-terminus of SopA.
Molecular dynamics simulations point out that the anticipated amphipathic helical motif of SopA has weak affinity for membranes. Furthermore, we experimentally present that SopA can affiliate with bacterial membranes, is detectable within the membrane fractions of bacterial lysates, and is delicate to the membrane potential. Additional, not like the wild-type SopA, a deletion of the C-terminal 29 amino acids leads to the lack of F plasmids from bacterial cells.
Novel lipid mixture for supply of plasmid DNA to immune cells within the spleen
This research experiences on the event of a novel lipid mixture that allows the environment friendly and extremely selective supply of plasmid DNA (pDNA) to immune cells within the spleen. Utilizing DODAP, an ionizable lipid that was beforehand considered inefficient for gene supply, we present for the primary time, that this ignored lipid will be efficiently used for environment friendly and focused gene supply in vivo, however solely when mixed with DOPE, a particular helper lipid. Utilizing sure DODAP and DOPE ratios resulted within the formation of lipid nanoparticles (LNPs) with a ~ 1000-fold increased gene expression, and this expression was particular for the spleen, making it essentially the most spleen-selective system for transfection utilizing pDNA.
The developed DODAP/DOPE-LNPs to focus on immune cells within the spleen through receptors for complement C3 and this pathway is essential for environment friendly gene expression. We hypothesize that the excessive spleen transfection exercise of DODAP/DOPE-LNPs is attributable to the promotion of gene expression related to B cell activation through complement receptors.
LNPs encapsulating tumor-antigen encoding pDNA confirmed each prophylactic and therapeutic anti-tumor results. The optimized LNPs resulted within the manufacturing of various cytokines and antigen-specific antibodies in addition to exerting antigen-specific cytotoxic results. This research revives using DODAP in gene supply and highlights the significance of utilizing applicable lipid mixtures for delivering genes to particular cells.
Dilemma on plasmid DNA purification: binding capability vs selectivity
Plasmid DNA chromatography is a robust discipline in fixed improvement and evolution. The usage of this system is taken into account necessary within the manufacturing of an environment friendly and protected formulation to be utilized for plasmid-mediated gene remedy. Regarding this, the seek for a really perfect chromatographic assist/ligand mixture motivated scientist to pursue a steady enchancment on the plasmid chromatography efficiency, in search of a development on the ligands and helps used.
 Mitochondria Isolation Kit |
|
AR0156 |
BosterBio |
1 kit |
EUR 106 |
 MicroRNA Isolation Kit |
|
KS341025 |
Biochain |
1 kit |
EUR 256 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Microsome Isolation Kit |
|
K249-50 |
Biovision |
|
EUR 528 |
 Preadipocyte Isolation Kit |
|
K583-5 |
Biovision |
|
EUR 533 |
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV200PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV205PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV220PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV225PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 Lipid Droplet Isolation Kit |
|
MET-5011 |
Cell Biolabs |
50 preps |
EUR 415 |
|
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer. |
 Total RNA Isolation Kit |
|
P-9100 |
EpiGentek |
50 samples |
EUR 347.25 |
|
Description: The best epigenetics products |
 Cell Mitochondria Isolation Kit |
|
abx090630-50100tests |
Abbexa |
50-100 tests |
EUR 258 |
|
|
 Tissue Mitochondria Isolation Kit |
|
abx090631-50100assays |
Abbexa |
50-100 assays |
EUR 258 |
|
|
 Mitochondrial DNA Isolation Kit |
|
K2117-50 |
ApexBio |
50 assays |
EUR 599 |
 Genomic DNA Isolation Kit |
|
K2118-50 |
ApexBio |
50 assays |
EUR 321 |
 Apoptotic Cell Isolation Kit |
|
55R-1349 |
Fitzgerald |
30 units |
EUR 729 |
|
Description: Apoptotic Cell Isolation Kit for use in the research laboratory |
Mitochondrial DNA Isolation Kit |
|
55R-1361 |
Fitzgerald |
50 assays |
EUR 825 |
|
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory |
Genomic DNA Isolation Kit |
|
55R-1362 |
Fitzgerald |
50 assays |
EUR 385 |
|
Description: Genomic DNA Isolation Kit for use in the research laboratory |
 Cartilage RNA Isolation Kit |
|
K2031010 |
Biochain |
1 kit |
EUR 419 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Saliva DNA Isolation Kit |
|
K5011050 |
Biochain |
1 kit |
EUR 256 |
 Urine DNA Isolation Kit |
|
K5011150 |
Biochain |
1 kit |
EUR 256 |
 Stool DNA Isolation Kit |
|
K5011250 |
Biochain |
1 kit |
EUR 256 |
 Serum DNA Isolation Kit |
|
K5018100 |
Biochain |
1 kit |
EUR 360 |
) ExoPure? Isolation Kit (Urine) |
|
K1240-10 |
Biovision |
|
EUR 697 |
ExoPure? Isolation Kit (Urine) |
|
K1240-2 |
Biovision |
|
EUR 403 |
Apoptotic Cell Isolation Kit |
|
K258-30 |
Biovision |
|
EUR 468 |
 Yeast Mitochondria Isolation Kit |
|
K259-50 |
Biovision |
|
EUR 419 |
Mitochondrial DNA Isolation Kit |
|
K280-50 |
Biovision |
|
EUR 588 |
Genomic DNA Isolation Kit |
|
K281-50 |
Biovision |
|
EUR 272 |
 Yeast Nuclei Isolation Kit |
|
K289-50 |
Biovision |
|
EUR 392 |
 Isolation Kit) Lipopolysaccharide (LPS) Isolation Kit |
|
K438-10 |
Biovision |
|
EUR 278 |
 Rapid Plant RNA Isolation Kit |
|
PT4191 |
Bio Basic |
50Preps |
EUR 79.69 |
|
|
 FitAmp Gel DNA Isolation Kit |
|
P-1007 |
EpiGentek |
100 Samples |
EUR 270.4 |
|
Description: The best epigenetics products |
 FitAmp Urine DNA Isolation Kit |
|
P-1017 |
EpiGentek |
100 Samples |
EUR 395.1 |
|
Description: kits suitable for this type of research |
 ChromaFlash Chromatin Isolation & Shearing Kit |
|
P-2023 |
EpiGentek |
100 Preperations |
EUR 270.4 |
|
Description: kits suitable for this type of research |
) Total RNA Isolation Kit (Tissue) |
|
P-9102 |
EpiGentek |
50 samples |
EUR 347.25 |
|
Description: reagents widely cited |
) Total RNA Isolation Kit (Plant) |
|
P-9104 |
EpiGentek |
50 samples |
EUR 347.25 |
|
Description: kits suitable for this type of research |
 Circulating Nuleic Acid Isolation Kit |
|
FACFK-050 |
Favorgen |
50 preps |
EUR 491 |
Circulating Nuleic Acid Isolation Kit |
|
FACFK-100 |
Favorgen |
100 preps |
EUR 825 |
 Total RNA Isolation Kit II |
|
FATRS--001 |
Favorgen |
100 preps |
EUR 308 |
Total RNA Isolation Kit II |
|
FATRS--050 |
Favorgen |
50 preps |
EUR 216 |
 Easy3-Plant RNA Isolation Kit |
|
FYG502-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Easy3-Plant RNA Isolation Kit |
|
FYG502-500P |
Yeastern Biotech |
500 Preps |
Ask for price |
 Easy3-Total NucleicAcid Isolation Kit |
|
FYG504-100ML |
Yeastern Biotech |
100 ml |
Ask for price |
Easy3-Total NucleicAcid Isolation Kit |
|
FYG504-500ML |
Yeastern Biotech |
500 ml |
Ask for price |
) Exosome Isolation kit (for urine) |
|
P120 |
101Bio |
- |
Ask for price |
Exosome Isolation kit (for urine) |
|
P120L |
101Bio |
- |
Ask for price |
Exosome Isolation kit (for urine) |
|
P120S |
101Bio |
- |
Ask for price |
 Detergent-Free Nuclei Isolation Kit |
|
P524-2 |
101Bio |
- |
Ask for price |
Detergent-Free Nuclei Isolation Kit |
|
P524-20 |
101Bio |
- |
Ask for price |
Detergent-Free Nuclei Isolation Kit |
|
P524-80 |
101Bio |
- |
Ask for price |
) Lysosome Isolation Kit (20 tests) |
|
P534 |
101Bio |
- |
Ask for price |
 Mouse Brain Microvessel Isolation Kit |
|
P750-4 |
101Bio |
- |
Ask for price |
 Rapid Bacteria RNA Isolation Kit |
|
BS8625 |
Bio Basic |
50Preps |
EUR 79.69 |
|
|
 Rapid Blood RNA Isolation Kit |
|
BT4182 |
Bio Basic |
10Preps |
EUR 81.97 |
|
|
Rapid Blood RNA Isolation Kit |
|
BT4183 |
Bio Basic |
50Preps |
EUR 164.19 |
|
|
Rapid Blood RNA Isolation Kit |
|
BT4184 |
Bio Basic |
250preps |
EUR 506.75 |
|
|
 Apoptotic DNA Ladder Isolation Kit |
|
K2045-50 |
ApexBio |
50 assays |
EUR 502 |
 Exo-Urine EV Isolation Kit |
|
EXOU100A-1 |
SBI |
2 x 5 reactions |
EUR 551 |
 XCF Exosomal DNA Isolation Kit |
|
XCF200A-1 |
SBI |
20 rxn |
EUR 386 |
|
|
) ExoPure? Isolation Kit (Cell Media) |
|
K1237-10 |
Biovision |
|
EUR 664 |
ExoPure? Isolation Kit (Cell Media) |
|
K1237-2 |
Biovision |
|
EUR 392 |
) ExoPure? Isolation Kit (Serum, Plasma) |
|
K1238-10 |
Biovision |
|
EUR 697 |
ExoPure? Isolation Kit (Serum, Plasma) |
|
K1238-2 |
Biovision |
|
EUR 392 |
) ExoPure? Isolation Kit (Bio Fluids) |
|
K1241-10 |
Biovision |
|
EUR 697 |
ExoPure? Isolation Kit (Bio Fluids) |
|
K1241-2 |
Biovision |
|
EUR 403 |
Apoptotic DNA Ladder Isolation Kit |
|
K170-50 |
Biovision |
|
EUR 468 |
 EZExtract? Core Histone Isolation Kit |
|
K234-100 |
Biovision |
|
EUR 262 |
 Cell Surface Protein Isolation Kit |
|
K295-10 |
Biovision |
|
EUR 501 |
 Bacterial Genomic DNA Isolation Kit |
|
K309-100 |
Biovision |
|
EUR 376 |
 OrgFrontier? Plasma Membrane Isolation Kit |
|
K414-10 |
Biovision |
|
EUR 566 |
 Org Frontier? Chloroplast Isolation Kit |
|
K468-10 |
Biovision |
|
EUR 555 |
 Whole Blood DNA Isolation Kit |
|
K528-100 |
Biovision |
|
EUR 376 |
 Frit Kit |
|
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
|
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
 Column Packing Kit |
|
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
|
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
 PCR Mycoplasma Detection Kit |
|
M034-Kit |
TOKU-E |
Kit |
EUR 266 |
 Methylamp Coupled DNA Isolation & Modification Kit |
|
P-1002 |
EpiGentek |
40 Samples |
EUR 395.1 |
|
Description: The best epigenetics products |
 FitAmp Plasma/Serum DNA Isolation Kit |
|
P-1004 |
EpiGentek |
100 Samples |
EUR 347.25 |
|
Description: fast delivery possible |
) Soil DNA Isolation Mini Kit (50prep) |
|
FASOI-001 |
Favorgen |
50 preps |
EUR 158 |
) Soil DNA Isolation Mini Kit (100prep) |
|
FASOI-001-1 |
Favorgen |
100 preps |
EUR 201 |
) Soil DNA Isolation Midi Kit (24prep) |
|
FASOI-002 |
Favorgen |
24 preps |
EUR 216 |
) Soil RNA Isolation Mini Kit (50prep) |
|
FASRK-001 |
Favorgen |
50 preps |
EUR 150 |
) Soil RNA Isolation Mini Kit (100prep) |
|
FASRK-100 |
Favorgen |
100 preps |
EUR 187 |
 Rapid Fungal Genomic DNA Isolation Kit |
|
FT71415 |
Bio Basic |
50Preps |
EUR 76.64 |
|
|
 Easy3-Plant Genomic DNA Isolation Kit |
|
FYG503-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Easy3-Plant Genomic DNA Isolation Kit |
|
FYG503-500P |
Yeastern Biotech |
500 Preps |
Ask for price |
 MagIso CD3 Cell Isolation Kit, Mouse |
|
WHK-B009 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD90.2 Cell Isolation Kit, Mouse |
|
WHK-B010 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso Neutrophil Cell Isolation Kit, Mouse |
|
WHK-B015 |
Creative Diagnostics |
10 tests, 100 tests |
Ask for price |
 MagIso CX3CR1 Cell Isolation Kit, Mouse |
|
WHK-B017 |
Creative Diagnostics |
10 tests, 100 tests |
Ask for price |
) Exosome Isolation kit (for serum & plasma) |
|
P101 |
101Bio |
- |
Ask for price |
Exosome Isolation kit (for serum & plasma) |
|
P101L |
101Bio |
- |
Ask for price |
Exosome Isolation kit (for serum & plasma) |
|
P101S |
101Bio |
- |
Ask for price |
) Exosome Isolation kit (forHuman Body Fluid) |
|
P121 |
101Bio |
- |
Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
|
P121L |
101Bio |
- |
Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
|
P121S |
101Bio |
- |
Ask for price |
) Mitochondria Isolation Kit (Mammalian cells / tissues) |
|
P507L |
101Bio |
- |
Ask for price |
 10-min. Single Cell Isolation Kit |
|
P713-20 |
101Bio |
- |
Ask for price |
 Rapid Bacteria Genomic DNA Isolation Kit |
|
BS8225 |
Bio Basic |
50Preps |
EUR 76.64 |
|
|
 Mammalian Mitochondria Isolation Kit for Tissue |
|
abx098857-50rxns |
Abbexa |
50 rxns |
EUR 411 |
|
|
 Broad Range Total RNA Isolation Kit |
|
K1341050 |
Biochain |
1 kit |
EUR 266 |
|
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
 Blood and Serum DNA Isolation Kit |
|
K5017100 |
Biochain |
1 kit |
EUR 360 |
 FastPure Bacteria DNA Isolation Mini Kit |
|
DC103 |
Vazyme |
100 rxn |
EUR 177 |
 FastPure Plant DNA Isolation Mini Kit |
|
DC104 |
Vazyme |
50 rxn |
EUR 160 |
 FastPure FFPE DNA Isolation Mini Kit |
|
DC105 |
Vazyme |
50 rxn |
EUR 201 |
) ExoPure? Isolation Kit (Stem Cell Media) |
|
K1239-10 |
Biovision |
|
EUR 751 |
ExoPure? Isolation Kit (Stem Cell Media) |
|
K1239-2 |
Biovision |
|
EUR 425 |
 Plant Tissue Genomic DNA Isolation Kit |
|
K316-100 |
Biovision |
|
EUR 376 |
 Human Whole Blood Monocyte Isolation Kit |
|
K933-30 |
Biovision |
|
EUR 376 |
 Mammalian Cell Genomic DNA Isolation Kit |
|
K967-100 |
Biovision |
|
EUR 381 |
 Albumin, cold alcohol isolation |
|
A0903 |
Bio Basic |
1g |
EUR 83.06 |
|
|
 cfMAX cfDNA Isolation System |
|
CFMAX100A-1 |
SBI |
1 Kit |
EUR 479 |
|
|
 RNA-easy Isolation Reagent |
|
R701-01 |
Vazyme |
100 ml |
EUR 168 |
RNA-easy Isolation Reagent |
|
R701-02 |
Vazyme |
200 ml |
EUR 212 |
 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
|
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
|
|
 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|
CAS510A-KIT |
SBI |
1 Kit |
EUR 805 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS750A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS770A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS790A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
|
CAS9LIG-KIT |
SBI |
1 Kit |
EUR 153 |
|
|
 FitAmp General Tissue Section DNA Isolation Kit |
|
P-1003 |
EpiGentek |
100 Samples |
EUR 628.55 |
|
Description: kits suitable for this type of research |
 FitAmp Paraffin Tissue Section DNA Isolation Kit |
|
P-1009 |
EpiGentek |
100 Samples |
EUR 585.05 |
|
Description: Ask the seller for details |
 EpiQuik Circulating Cell-Free DNA Isolation Kit |
|
P-1064 |
EpiGentek |
50 Isolations |
EUR 347.25 |
|
Description: kits suitable for this type of research |
) Soil DNA Plus Isolation Mini Kit (50prep) |
|
FAPSO-001 |
Favorgen |
50 preps |
EUR 158 |
) Soil DNA Plus Isolation Mini Kit (100prep) |
|
FAPSO-001-1 |
Favorgen |
100 preps |
EUR 201 |
 MagIso Hematopoietic Progenitor Cell Isolation Kit, Mouse |
|
WHK-B005 |
Creative Diagnostics |
10 tests |
EUR 604 |
 MagIso Hematopoietic Progenitor Cell Isolation Kit, Human |
|
WHK-B008 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso Pan Dendritic Cell Isolation Kit, Mouse |
|
WHK-B011 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso Pan B Cell Isolation Kit, Mouse |
|
WHK-B013 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
MagIso Hematopoietic Progenitor Cell Isolation Kit, Mouse |
|
WHK-B016 |
Creative Diagnostics |
10 tests, 100 tests |
Ask for price |
 MagIso Pan Monocyte Cell Isolation Kit, Human |
|
WHK-B021 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso Pan B Cell Isolation Kit, Human |
|
WHK-B022 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso Naïve B Cell Isolation Kit, Human |
|
WHK-B024 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD14+ Monocytes Cell Isolation Kit, Human |
|
WHK-B029 |
Creative Diagnostics |
10 tests, 100 tests |
Ask for price |
) Exosome Isolation kit (for cell culture media) |
|
P100 |
101Bio |
- |
Ask for price |
Exosome Isolation kit (for cell culture media) |
|
P100L |
101Bio |
- |
Ask for price |
Exosome Isolation kit (for cell culture media) |
|
P100S |
101Bio |
- |
Ask for price |
 8-min Intact Plant Chloroplast Isolation Kit |
|
P511L |
101Bio |
- |
Ask for price |
 Dissociation / Single Cell Isolation Kit) Tissue (Tumor) Dissociation / Single Cell Isolation Kit |
|
P712-25 |
101Bio |
- |
Ask for price |
Tissue (Tumor) Dissociation / Single Cell Isolation Kit |
|
P712-4 |
101Bio |
- |
Ask for price |
Tissue (Tumor) Dissociation / Single Cell Isolation Kit |
|
P712-50 |
101Bio |
- |
Ask for price |
 One-Tube Bacteria Genomic DNA Isolation Kit |
|
BS8413 |
Bio Basic |
100preps |
EUR 78.93 |
|
|
One-Tube Bacteria Genomic DNA Isolation Kit |
|
BS8414 |
Bio Basic |
500Preps, 500prep |
EUR 165.71 |
|
|
 Mammalian Mitochondria Isolation Kit for Cultured Cells |
|
20-abx098856 |
Abbexa |
|
|
|
|
 FastPure Cell/Tissue DNA Isolation Mini Kit |
|
DC102 |
Vazyme |
100 rxn |
EUR 177 |
 FastPure Blood DNA Isolation Mini Kit V2 |
|
DC111-01 |
Vazyme |
50 rxn |
EUR 179 |
FastPure Blood DNA Isolation Mini Kit V2 |
|
DC111-02 |
Vazyme |
200 rxn |
EUR 394 |
 Human Whole Blood Polymorphonuclear Cell Isolation Kit |
|
K483-30 |
Biovision |
|
EUR 376 |
 FastPure Cell/Tissue Total RNA Isolation Kit |
|
RC101 |
Vazyme |
50 rxn |
EUR 229 |
) PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|
PIN320A-KIT |
SBI |
1 Kit |
EUR 4941 |
|
|
) PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|
PIN340iPS-KIT |
SBI |
1 Kit |
EUR 4941 |
|
|
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|
GE620A-KIT |
SBI |
1 kit |
EUR 2132 |
|
|
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|
GE622A-KIT |
SBI |
1 kit |
EUR 2132 |
|
|
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|
GE624A-KIT |
SBI |
1 kit |
EUR 2132 |
|
|
 MagIso Pan B Cell Isolation Kit, Mouse Ⅱ |
|
WHK-B012 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 Cell Isolation Kit, Mouse) MagIso Natural killer (NK) Cell Isolation Kit, Mouse |
|
WHK-B014 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD8 Naïve T Cell Isolation Kit, Mouse |
|
WHK-B018 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD4 Naïve T Cell Isolation Kit, Mouse |
|
WHK-B019 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD8 Naïve T Cell Isolation Kit, Human |
|
WHK-B025 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD8 Memory T Cell Isolation Kit, Human |
|
WHK-B026 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD4 Naïve T Cell Isolation Kit, Human |
|
WHK-B027 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 MagIso CD4 Memory T Cell Isolation Kit, Human |
|
WHK-B028 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
 Cell Isolation Kit, Human) MagIso B Cell (CD43-) Cell Isolation Kit, Human |
|
WHK-B030 |
Creative Diagnostics |
20 tests, 200 tests |
Ask for price |
) Exosome Isolation kit (for stem cell culture media) |
|
P107 |
101Bio |
- |
Ask for price |
Exosome Isolation kit (for stem cell culture media) |
|
P107S |
101Bio |
- |
Ask for price |
 Nuclei and Cytosol Isolation Kit for Adipose Tissues |
|
P529 |
101Bio |
- |
Ask for price |
 Easy-Exo Saliva Exosome Isolation Kit, 95% pure |
|
P530 |
101Bio |
- |
Ask for price |
Easy-Exo Saliva Exosome Isolation Kit, 95% pure |
|
P530S |
101Bio |
- |
Ask for price |
 Isolation Kit) Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit |
|
W2603-10 |
101Bio |
- |
Ask for price |
Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit |
|
W2603-20 |
101Bio |
- |
Ask for price |
Hipro Circulating Cell-Free DNA (cfDNA) Isolation Kit |
|
W2603-50 |
101Bio |
- |
Ask for price |
) CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps) |
|
CBA-155 |
Cell Biolabs |
5 preps |
EUR 798 |
|
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis. |
The current evaluation explores the totally different approaches used over time to purify plasmid DNA, ambitioning each excessive restoration and excessive purity ranges. Total, it’s introduced a essential dialogue counting on the relevance of the binding capability versus selectivity of the helps.
