A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines

Aedes (Ae.) aegypti and Ae. albopictus mosquitoes transmit arthropod-borne illnesses across the globe, inflicting ~ 700,000 deaths annually. Genetic mutants are invaluable instruments to interrogate each basic vector biology and mosquito host elements essential for viral an infection. Nevertheless, only a few genetic mutants have been described in mosquitoes compared to mannequin organisms. The relative ease of making use of CRISPR/Cas9-based gene enhancing has remodeled genome engineering and has quickly elevated the variety of out there gene mutants in mosquitoes.

But, in vivo research is probably not sensible for screening massive units of mutants or doable for laboratories that lack insectaries. Thus, it could be helpful to adapt CRISPR/Cas9 techniques to widespread mosquito cell traces. On this examine, we generated and characterised a mosquito optimized, plasmid-based CRISPR/Cas9 system to be used in U4.4 (Ae. albopictus) and Aag2 (Ae. aegypti) cell traces. We demonstrated extremely environment friendly enhancing of the AGO1 locus and remoted U4.Four and Aag2 cell traces with lowered AGO1 expression. Additional, we used homology-directed restore to ascertain knock-in Aag2 cell traces with a 3xFLAG-tag on the N-terminus of endogenous AGO1. These experimentally verified plasmids are versatile, cost-effective, and effectively edit immune competent mosquito cell traces which can be extensively utilized in arbovirus research.

A statistical framework for dedication of minimal plasmid copy quantity required for transgene expression in mammalian cells

Plasmid DNA (pDNA) has been extensively used for non-viral gene supply. After pDNA molecules enter a mammalian cell, they might be trapped in subcellular constructions or degraded by nucleases. Solely a fraction of them can perform as templates for transcription within the nucleus. Thus, an essential query is, what’s the minimal quantity of pDNA molecules that should be delivered right into a cell for transgene expression? At current, it’s technically a problem to experimentally reply the query.

To this finish, we developed a statistical framework to ascertain the connection between two experimentally quantifiable elements – common copy variety of pDNA per cell amongst a gaggle of cells after transfection and p.c of the cells with transgene expression. The framework was utilized to the evaluation of electrotransfection underneath totally different experimental situations in vitro. We experimentally diverse the typical copy quantity per cell and the electrotransfection effectivity via modifications in extracellular pDNA dose, electrical subject power, and pulse quantity.

The experimental knowledge might be defined or predicted quantitatively by the statistical framework. Based mostly on the information and the framework, we may predict that the minimal variety of pDNA molecules within the nucleus for transgene expression was on the order of 10. Though the prediction was depending on the cell and experimental situations used within the examine, the framework could also be typically utilized to evaluation of non-viral gene supply.

Categorical in Vitro Plasmid Transfection Achieved with 16 ⁺ Uneven Peptide Dendrimers

Uneven cationic amino acid-based dendrimers are extremely branched chemically derived gene vectors developed to move cargo akin to plasmid DNA throughout the plasma membrane. We’ve beforehand demonstrated their propensity to enter cells that type caveolae, pushed by constructive cost density and promoted by arginine head teams. Caveolae are plasma membrane subdomains serving plenty of mobile capabilities together with endocytosis.

Their formation requires membrane proteins (caveolins) and cytoplasmic proteins (cavins), in order that gene disruption of both caveolin-1 or cavin-1 (also referred to as PTRF, i.e., polymerase I and transcript launch issue) leads to caveola deficiency. Right here we evaluated the flexibility of a 16⁺ charged uneven arginine dendrimer to transfect plasmid DNA into cultured cells.

We unveiled environment friendly transfection efficiencies (≥30%) 24-48 h after exposing the cells to dendrimer/pDNA complexes for less than 5 min. Utilizing wild kind (WT) and caveolin-1 or PTRF gene-disrupted, i.e., caveola-deficient mouse embryo fibroblasts, we additional present that caveolae promote pDNA transfection by 16⁺ charged uneven arginine dendrimers.

Construction-Exercise Relationship of Mono-Ion Complexes for Plasmid DNA Supply by Muscular Injection

The structure-activity relationship of mono-ion complexes (MICs) for plasmid DNA (pDNA) supply by muscular injection is demonstrated. MICs have been shaped between pDNA and monocationic poly(ethylene glycol) (PEG) macromolecules. As monocationic PEGs, the ω-amide-pentylimidazolium (APe-Im) end-modified PEGs with a secure amide (Am) and hydrolytic ester (Es) bond, that’s, APe-Im-Am-PEG and APe-Im-Es-PEG, respectively, are synthesized.

The distinction between the APe-Im-Am-PEG and APe-Im-Es-PEG was solely a spacer construction between a terminal cation and a PEG chain. The ensuing pDNA MICs with APe-Im-Am-PEG at a cost ratio (+/-) of 32 or 64 have been extra secure than these with APe-Im-Es-PEG within the presence of serum proteins. The best gene expression by muscular injection was achieved utilizing the APe-Im-Am-PEG/pDNA MIC at a cost ratio (+/-) of 32 with a smaller particle diameter of roughly 50 nm, as in comparison with that cost ratio of 64. Consequently, the pDNA MIC with the monocationic PEG with a secure amide spacer, as in comparison with a hydrolytic ester spacer, is taken into account to be appropriate for the best gene expression by muscular injection.

pOET3 transfer plasmid
200104	Oxford Expression Technologies	10 µg	EUR 208.32

pOET4 transfer plasmid
200105	Oxford Expression Technologies	10 µg	EUR 208.32

pOET5.1 transfer plasmid
200106	Oxford Expression Technologies	10 µg	EUR 208.32

pOET8.VE1 transfer plasmid
200121	Oxford Expression Technologies	10 µg	EUR 409.2

pOET8.VE2 transfer plasmid
200122	Oxford Expression Technologies	10 µg	EUR 409.2

pOET8.VE3 transfer plasmid
200123	Oxford Expression Technologies	10 µg	EUR 409.2

pOET9 CMV transfer plasmid
200133	Oxford Expression Technologies	10 µg	EUR 280.24

pOET9 EF1α transfer plasmid
200131	Oxford Expression Technologies	10 µg	EUR 280.24

pOET9 CCAG transfer plasmid
200132	Oxford Expression Technologies	10 µg	EUR 280.24

pOET9 SV40 transfer plasmid
200134	Oxford Expression Technologies	10 µg	EUR 280.24

pOET6 BacMAM transfer plasmid
200107	Oxford Expression Technologies	10 µg	EUR 208.32

6XHis azide
12628-1mg	AAT Bioquest	1 mg	EUR 295
Description: 6XHis azide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

6XHis alkyne
12629-1mg	AAT Bioquest	1 mg	EUR 295
Description: 6XHis alkyne is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

6XHis maleimide
12626-1mg	AAT Bioquest	1 mg	EUR 295
Description: 6XHis maleimide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

6XHis Succinimidyl Ester
12624-1mg	AAT Bioquest	1 mg	EUR 295
Description: 6XHis Succinimidyl Ester is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

pET- NLS- Cas9- 6xHis
PVT10639	Lifescience Market	2 ug	EUR 361.2

TransferTip ES cell transfer
E5195000079	Scientific Laboratory Supplies	PK25	EUR 727.2

100µL Transfer Pipettes
TP100	BioAssay Systems	N/A	EUR 15
Description: For pipetting 100µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, disposable, easy-to-use. Quantity: 10 pipettes.

1000µL Transfer Pipettes
TP1000	BioAssay Systems	N/A	EUR 15
Description: For pipetting 1000µL samples. To be used with the Histamine Quick Test Strips. Key Features: Single use, disposable, easy-to-use. Quantity: 10 pipettes.

20µL Transfer Pipettes
TP20	BioAssay Systems	N/A	EUR 15
Description: For pipetting 20µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, disposable, easy-to-use. Quantity: 10 pipettes.

200µL Transfer Pipettes
TP200	BioAssay Systems	N/A	EUR 15
Description: For pipetting 200µL samples. To be used with the Histamine Quick Test Strips. Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

300µL Transfer Pipettes
TP300	BioAssay Systems	N/A	EUR 15
Description: For pipetting 300µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

400µL Transfer Pipettes
TP400	BioAssay Systems	N/A	EUR 15
Description: For pipetting 400µL samples. To be used with the Quick Test Strips (e.g. D-Lactic Acid Quick Test Strips, L-Lactic Acid Quick Test Strips, Malic Acid Quick Test Strips). Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

50µL Transfer Pipettes
TP50	BioAssay Systems	N/A	EUR 15
Description: For pipetting 50µL samples. To be used with the Pyruvate Quick Test Strips. Key Features: Single use, dsposable, easy-to-use. Quantity: 10 pipettes.

Propane Transfer System
M-CEM-37015	MiTeGen	1 UNIT	EUR 688
Description: Propane Transfer System

Thermal Transfer Ribbon
HIS3233	Scientific Laboratory Supplies	EACH	EUR 181.2

WESTERN TRANSFER MODULE
IB95000	IBI Scientific	-	EUR 861.3

WESTERN TRANSFER SYSTEM,
IB95030	IBI Scientific	.2uM PVDF MEMBRANE, 10/PACK	EUR 221.2

Transfer Rack 0.5 mL
E3880000305	Scientific Laboratory Supplies	EACH	EUR 52.8

Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat cholest erolester transfer protein/lipid transfer protein ELISA kit
E02C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog cholest erolester transfer protein/lipid transfer protein ELISA kit
E08C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E07C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat cholest erolester transfer protein/lipid transfer protein ELISA kit
E06C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit cholest erolester transfer protein/lipid transfer protein ELISA kit
E04C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human cholest erolester transfer protein/lipid transfer protein ELISA kit
E01C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Human cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse cholest erolester transfer protein/lipid transfer protein ELISA kit
E03C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey cholest erolester transfer protein/lipid transfer protein ELISA kit
E09C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

CAPS Transfer Buffer 5X
21420016-1	Bio-WORLD	500 mL	EUR 60.54
Description: 3- (Cyclohexylamino) -1-propanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid

CAPS Transfer Buffer 5X
21420016-2	Bio-WORLD	1 L	EUR 91.52
Description: 3- (Cyclohexylamino) -1-propanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid

SPINE Puck Transfer Tool
M-SP-PTT-1	MiTeGen	1 UNIT	EUR 386
Description: SPINE Puck Transfer Tool

CAPS Transfer Buffer 10X
20960009-1	Bio-WORLD	100 mL	EUR 26.06
Description: 3- (Cyclohexylamino) -1-propanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid

CAPS Transfer Buffer 10X
20960009-2	Bio-WORLD	500 mL	EUR 78.08
Description: 3- (Cyclohexylamino) -1-propanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid

CAPS Transfer Buffer 10X
20960009-3	Bio-WORLD	1 L	EUR 148.28
Description: 3- (Cyclohexylamino) -1-propanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid

6xHis-T2A-Puro-pA OmniTag Minicircle Donor
OT503MC-1	SBI	10 µg	EUR 709

Phospholipid transfer Antibody
20-abx110096	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Rapid Transfer Buffer 10X
10230000-1	Bio-WORLD	1 L	Ask for price

Rapid Transfer Buffer 10X
10230000-2	Bio-WORLD	4 L	Ask for price

Transfer Rack 1.5/2.0 mL
E3880000151	Scientific Laboratory Supplies	EACH	EUR 49.2

Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E05C0732-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E05C0732-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig cholest erolester transfer protein/lipid transfer protein ELISA kit
E05C0732-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig cholest erolester transfer protein/lipid transfer protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

6400 Thermal Transfer Ribbon
804777	Scientific Laboratory Supplies	EACH	EUR 45.72

Monoclonal Anti-Poly-His (6xhis) (His-tag) IgG
HISP14-M	Alpha Diagnostics	100 ug	EUR 578.4

WESTERN TRANSFER SYSTEM, THIN
IB95010	IBI Scientific	BLOT PAPER, 0.38MM - 10/PACK	EUR 38.22

SSRL Cassette Transfer Handle
M-CP-111-003	MiTeGen	1 UNIT	EUR 460
Description: SSRL Cassette Transfer Handle

Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit
201-12-1516	SunredBio	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit
GA-E1532HM-48T	GenAsia Biotech	48T	EUR 346.8

Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit
GA-E1532HM-96T	GenAsia Biotech	96T	EUR 559.2

Human cholest erolester transfer protein/lipid transfer protein(CETP/LTP)ELISA Kit
QY-E03917	Qayee Biotechnology	96T	EUR 433.2

Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit
YLA0018HU-48T	Shanghai YL Biotech	48T	EUR 435

Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit
YLA0018HU-96T	Shanghai YL Biotech	96T	EUR 562.5

Human cholest erolester transfer protein/lipid transfer protein,CETP/LTP ELISA Kit
SL0449Hu	Sunlong	96 Tests	EUR 468

Cryo Transfer Face for SEM
M-CEM-37000-S	MiTeGen	1 UNIT	EUR 3295
Description: Cryo Transfer Face for SEM

Cryo Transfer Face for TEM
M-CEM-37000-T	MiTeGen	1 UNIT	EUR 3185
Description: Cryo Transfer Face for TEM

Alkaline transfer solution (10X)
10750000-1	Bio-WORLD	1 L	EUR 68.63

Phospholipid transfer Antibody (HRP)
20-abx108612	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Thermal Transfer Ribbon 1x2.7 OD
HIS3524	Scientific Laboratory Supplies	EACH	EUR 244.8

Bis-Tris Transfer Buffer 20X
21420066-1	Bio-WORLD	1 L	EUR 114.99

Bis-Tris Transfer Buffer 20X
21420066-2	Bio-WORLD	2 L	EUR 157.04

Phospholipid transfer Antibody (FITC)
20-abx107192	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Laboratory Thermal Transfer Printer
BAL4730	Scientific Laboratory Supplies	EACH	EUR 1458

Human Transfer factor ELISA Kit
ELA-E1333h	Lifescience Market	96 Tests	EUR 988.8

BioPhotometer data transfer software
E6132854007	Scientific Laboratory Supplies	EACH	EUR 464.4

Goat Anti-Poly-His (6xhis) (His-tag) IgG, aff pure
HISP16-A	Alpha Diagnostics	100 ug	EUR 489.6

Transfer Pipette 15mL Large Bulb
ARG1836	Scientific Laboratory Supplies	PK250	EUR 34.34

Xpert Native Transfer Buffer(10X)
P9001-050	GenDepot	500ml	EUR 111.6

Xpert Native Transfer Buffer(10X)
P9001-100	GenDepot	1L	EUR 165.6

Fast Western Transfer Buffer Kit
20810005-1	Bio-WORLD	1 Kit	EUR 128.98
Description: EzFastBlo

Tris Glycine Transfer Buffer 10X
20960006-1	Bio-WORLD	1 L	EUR 66.99

Tris Glycine Transfer Buffer 10X
20960006-2	Bio-WORLD	4 L	EUR 173.72

Tris Glycine Transfer Buffer 10X
20960006-3	Bio-WORLD	500 mL	EUR 46.97

Phospholipid transfer Antibody (Biotin)
20-abx105774	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

PAGE Gel Transfer Buffer, 10 x
GR103018	Genorise Scientific	1 L	EUR 79

Tris Glycine Transfer Buffer Packs
42020403-1	Bio-WORLD	40 PK (s)	EUR 109.86

Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged
IFRIL6YSTNT6XHIS1000ug	Innovative research	each	EUR 5756
Description: Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged

Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged
IFRIL6YSTNT6XHIS200ug	Innovative research	each	EUR 3198
Description: Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged

Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged
IFRIL6YSTNT6XHIS500ug	Innovative research	each	EUR 3627
Description: Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged

Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged
IFRIL6YSTNT6XHIS50ug	Innovative research	each	EUR 2348
Description: Ferret IL-6 Recombinant Yeast N-Terminal 6xHis Tagged

FastBlot Transfer Buffer 5X, pH 11.0
40320062-1	Bio-WORLD	500 mL	EUR 41.3

Semi Dry Blot Transfer Buffer 10X
21420067-1	Bio-WORLD	1 L	EUR 32.46

Semi Dry Blot Transfer Buffer 10X
21420067-2	Bio-WORLD	2 L	EUR 63.14

Phospholipid transfer Polyclonal Antibody
42297-100ul	SAB	100ul	EUR 399.6

SuperBlot Western Transfer buffer 10X
DG-WEST	Bio Basic	500ml	EUR 136.56

Human Transfer factor,TF ELISA Kit
201-12-1733	SunredBio	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Transfer factor,TF ELISA Kit
CN-04584H1	ChemNorm	96T	EUR 520.8

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