A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines

Aedes (Ae.) aegypti and Ae. albopictus mosquitoes transmit arthropod-borne illnesses across the globe, inflicting ~ 700,000 deaths annually. Genetic mutants are invaluable instruments to interrogate each basic vector biology and mosquito host elements essential for viral an infection. Nevertheless, only a few genetic mutants have been described in mosquitoes compared to mannequin organisms. The relative ease of making use of CRISPR/Cas9-based gene enhancing has remodeled genome engineering and has quickly elevated the variety of out there gene mutants in mosquitoes.

But, in vivo research is probably not sensible for screening massive units of mutants or doable for laboratories that lack insectaries. Thus, it could be helpful to adapt CRISPR/Cas9 techniques to widespread mosquito cell traces. On this examine, we generated and characterised a mosquito optimized, plasmid-based CRISPR/Cas9 system to be used in U4.4 (Ae. albopictus) and Aag2 (Ae. aegypti) cell traces. We demonstrated extremely environment friendly enhancing of the AGO1 locus and remoted U4.Four and Aag2 cell traces with lowered AGO1 expression. Additional, we used homology-directed restore to ascertain knock-in Aag2 cell traces with a 3xFLAG-tag on the N-terminus of endogenous AGO1. These experimentally verified plasmids are versatile, cost-effective, and effectively edit immune competent mosquito cell traces which can be extensively utilized in arbovirus research.

A statistical framework for dedication of minimal plasmid copy quantity required for transgene expression in mammalian cells

Plasmid DNA (pDNA) has been extensively used for non-viral gene supply. After pDNA molecules enter a mammalian cell, they might be trapped in subcellular constructions or degraded by nucleases. Solely a fraction of them can perform as templates for transcription within the nucleus. Thus, an essential query is, what’s the minimal quantity of pDNA molecules that should be delivered right into a cell for transgene expression? At current, it’s technically a problem to experimentally reply the query.

To this finish, we developed a statistical framework to ascertain the connection between two experimentally quantifiable elements – common copy variety of pDNA per cell amongst a gaggle of cells after transfection and p.c of the cells with transgene expression. The framework was utilized to the evaluation of electrotransfection underneath totally different experimental situations in vitro. We experimentally diverse the typical copy quantity per cell and the electrotransfection effectivity via modifications in extracellular pDNA dose, electrical subject power, and pulse quantity.

The experimental knowledge might be defined or predicted quantitatively by the statistical framework. Based mostly on the information and the framework, we may predict that the minimal variety of pDNA molecules within the nucleus for transgene expression was on the order of 10. Though the prediction was depending on the cell and experimental situations used within the examine, the framework could also be typically utilized to evaluation of non-viral gene supply.

A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines

Categorical in Vitro Plasmid Transfection Achieved with 16 + Uneven Peptide Dendrimers

Uneven cationic amino acid-based dendrimers are extremely branched chemically derived gene vectors developed to move cargo akin to plasmid DNA throughout the plasma membrane. We’ve beforehand demonstrated their propensity to enter cells that type caveolae, pushed by constructive cost density and promoted by arginine head teams. Caveolae are plasma membrane subdomains serving plenty of mobile capabilities together with endocytosis.

Their formation requires membrane proteins (caveolins) and cytoplasmic proteins (cavins), in order that gene disruption of both caveolin-1 or cavin-1 (also referred to as PTRF, i.e., polymerase I and transcript launch issue) leads to caveola deficiency. Right here we evaluated the flexibility of a 16+ charged uneven arginine dendrimer to transfect plasmid DNA into cultured cells.

We unveiled environment friendly transfection efficiencies (≥30%) 24-48 h after exposing the cells to dendrimer/pDNA complexes for less than 5 min. Utilizing wild kind (WT) and caveolin-1 or PTRF gene-disrupted, i.e., caveola-deficient mouse embryo fibroblasts, we additional present that caveolae promote pDNA transfection by 16+ charged uneven arginine dendrimers.

Construction-Exercise Relationship of Mono-Ion Complexes for Plasmid DNA Supply by Muscular Injection

The structure-activity relationship of mono-ion complexes (MICs) for plasmid DNA (pDNA) supply by muscular injection is demonstrated. MICs have been shaped between pDNA and monocationic poly(ethylene glycol) (PEG) macromolecules. As monocationic PEGs, the ω-amide-pentylimidazolium (APe-Im) end-modified PEGs with a secure amide (Am) and hydrolytic ester (Es) bond, that’s, APe-Im-Am-PEG and APe-Im-Es-PEG, respectively, are synthesized.

The distinction between the APe-Im-Am-PEG and APe-Im-Es-PEG was solely a spacer construction between a terminal cation and a PEG chain. The ensuing pDNA MICs with APe-Im-Am-PEG at a cost ratio (+/-) of 32 or 64 have been extra secure than these with APe-Im-Es-PEG within the presence of serum proteins. The best gene expression by muscular injection was achieved utilizing the APe-Im-Am-PEG/pDNA MIC at a cost ratio (+/-) of 32 with a smaller particle diameter of roughly 50 nm, as in comparison with that cost ratio of 64. Consequently, the pDNA MIC with the monocationic PEG with a secure amide spacer, as in comparison with a hydrolytic ester spacer, is taken into account to be appropriate for the best gene expression by muscular injection.

Antitumor and antioxidant actions of purple potato ethanolic extract and its interplay with liposomes, albumin and plasmid DNA

The intention of the examine was to broadly decide the organic actions of purple potato ethanolic extract of the Blue Congo selection (BCE). The antioxidant exercise of BCE was decided in relation to liposome membranes, and peroxidation was induced by UVB and AAPH. To make clear the antioxidant exercise of BCE, we investigated its interactions with hydrophilic and hydrophobic areas of a membrane utilizing fluorimetric and FTIR strategies. Subsequent, we investigated the cytotoxicity and pro-apoptotic actions of BCE in two human colon most cancers cell traces (HT-29 and Caco-2) and in regular cells (IPEC-J2).
As well as, the flexibility to inhibit enzymes which can be concerned in pro-inflammatory reactions was examined. Moreover, BCE interactions with serum albumin and plasmid DNA have been investigated utilizing regular state fluorescence spectroscopy and a single molecule fluorescence approach (TCSPC-FCS). We proved that BCE successfully protects lipid membranes towards the method of peroxidation and efficiently inhibits the cyclooxygenase and lipoxygenase enzymes.

Human Antigen KI-67 (MKI67)

1-CSB-RP116174h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Antigen KI-67(MKI67) ,partial expressed in E.coli

Helicobacter pylori Vacuolating cytotoxin autotransporter (vacA)

1-CSB-RP143794Ba(C)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Helicobacter pylori Vacuolating cytotoxin autotransporter(vacA),partial expressed in E.coli

Human Glutamyl aminopeptidase (ENPEP)

1-CSB-RP147594h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Glutamyl aminopeptidase(ENPEP),partial expressed in E.coli

Saccharomyces cerevisiae Trehalose-phosphatase (TPS2)

1-CSB-RP165694Ye(c)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Saccharomyces cerevisiae Trehalose-phosphatase(TPS2),partial expressed in E.coli

Mouse Protein Muc5ac (Muc5ac)

1-CSB-RP172294m(C)
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Protein Muc5ac(Muc5ac),partial expressed in E.coli

Human Histone deacetylase 7 (HDAC7)

1-CSB-RP178994h(C)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Histone deacetylase 7(HDAC7),partial expressed in E.coli

Human Telomerase protein component 1 (TEP1)

1-CSB-RP180494h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Telomerase protein component 1(TEP1),partial expressed in E.coli

Human Collagen alpha-1 (XVII) chain (COL17A1)

1-CSB-RP180994h(c)
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Collagen alpha-1(XVII) chain(COL17A1),partial expressed in E.coli

Saccharomyces cerevisiae Neutral trehalase (NTH1)

1-CSB-RP181594Ye(c)
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Saccharomyces cerevisiae Neutral trehalase(NTH1),partial expressed in E.coli

pET-(-30)GFP-9xGGS-Cre-6xHis Plasmid

PVT17107 2 ug
EUR 390

SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag

E80027
  • EUR 635.80
  • EUR 4087.60
  • 1 ml
  • 100 ul

Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial

E80028
  • EUR 388.30
  • EUR 860.20
  • 20 ul
  • 100 ul

Recombinant Thermobifida Fusca Cutinase(C-6xHis)

CR10-10ug 10ug
EUR 242.4
Description: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.

Recombinant Thermobifida Fusca Cutinase(C-6xHis)

CR10-1mg 1mg
EUR 2983.2
Description: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.

Recombinant Thermobifida Fusca Cutinase(C-6xHis)

CR10-500ug 500ug
EUR 2106
Description: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.

Recombinant Thermobifida Fusca Cutinase(C-6xHis)

CR10-50ug 50ug
EUR 595.2
Description: Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl,1mM 2-mercaptoethanol,2mM MnCl2,150mM NaCl,pH7.5.

SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag

E80024
  • EUR 635.80
  • EUR 4995.10
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion

E80026
  • EUR 588.50
  • EUR 823.90
  • 20 ul
  • 50 ul

SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag

E80021
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag

E80023
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag

E80020
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag

E80022
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag

E80025
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

pLenti-CARM1 shRNA-2 Plasmid

PVTBAV03691-2 2 ug
EUR 427.2

pLenti-CTLA4 shRNA-2 Plasmid

PVTBAV05689-2 2 ug
EUR 427.2

pLenti-FOXM1 shRNA-2 Plasmid

PVTBAV08732-2 2 ug
EUR 427.2

pLenti-JUN shRNA-2 Plasmid

PVTBAV11741-2 2 ug
EUR 427.2

pLenti-LHX6 shRNA-2 Plasmid

PVTBAV12881-2 2 ug
EUR 427.2

pLenti-MAGEA3 shRNA-2 Plasmid

PVTBAV13661-2 2 ug
EUR 427.2

pLenti-RUNX3 shRNA-2 Plasmid

PVTBAV20583-2 2 ug
EUR 427.2

pLenti-Slc7a11 shRNA-2 Plasmid

PVTBAV21973-2 2 ug
EUR 427.2

pLenti-STAT3 shRNA-2 Plasmid

PVTBAV22921-2 2 ug
EUR 427.2

pLenti-XRCC5 shRNA-2 Plasmid

PVTBAV26238-2 2 ug
EUR 427.2

pET-dCas9-VP64-6xHis

PVT17702 2 ug
EUR 360

pET-6xHis-(-30)GFP

PVT17881 2 ug
EUR 409.2

pET- NLS- Cas9- 6xHis

PVT10639 2 ug
EUR 361.2

Recombinant Transmembrane Protease Serine 2 Protein, Partial

E80016
  • EUR 470.80
  • EUR 753.50
  • EUR 1824.90
  • 20 ul
  • 100 ul
  • 500 ul

ACE2, His-Tag Protein

E80019
  • EUR 612.70
  • EUR 1437.70
  • 20 ul
  • 100 ul

Mouse Anti-6xHIS Tag monoclonal antibody

CABT-BL8767 100ug
EUR 670.8

Anti-6xHIS Tag Antibody | CHIS-45A

CHIS-45A 1.0 mg
EUR 1142
Description:

Anti-6xHIS Tag Antibody | CHIS-45A | Immunology Consultants Laboratory

Host: Chicken

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | CHIS-45F

CHIS-45F 1.0 mg
EUR 1157
Description:

Anti-6xHIS Tag Antibody | CHIS-45F | Immunology Consultants Laboratory

Host: Chicken

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | CHIS-45P

CHIS-45P 1.0 mg
EUR 1173
Description:

Anti-6xHIS Tag Antibody | CHIS-45P | Immunology Consultants Laboratory

Host: Chicken

Format: HRP Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | MHIS-45A

MHIS-45A 1.0 mg
EUR 1142
Description:

Anti-6xHIS Tag Antibody | MHIS-45A | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-6xHIS Tag Antibody | RHIS-45A

RHIS-45A 1.0 mg
EUR 1142
Description:

Anti-6xHIS Tag Antibody | RHIS-45A | Immunology Consultants Laboratory

Host: Rabbit

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | RHIS-45B

RHIS-45B 1.0 mg
EUR 1157
Description:

Anti-6xHIS Tag Antibody | RHIS-45B | Immunology Consultants Laboratory

Host: Rabbit

Format: Biotin Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | RHIS-45F

RHIS-45F 1.0 mg
EUR 1157
Description:

Anti-6xHIS Tag Antibody | RHIS-45F | Immunology Consultants Laboratory

Host: Rabbit

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | RHIS-45P

RHIS-45P 1.0 mg
EUR 1173
Description:

Anti-6xHIS Tag Antibody | RHIS-45P | Immunology Consultants Laboratory

Host: Rabbit

Format: HRP Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

pDONR223-CD73 Plasmid

PVTB00480-2 2 ug
EUR 427.2

Plasmid Midi Kit I

K1314-2
EUR 314.4

Plasmid Midi Kit II

K1315-2
EUR 314.4

Plasmid ezFilter Mega3 Kit

K1320-2
EUR 411.6

Plasmid ezFilter Mega6 Kit

K1321-2
EUR 444

Plasmid ezFilter Mega10 Kit

K1322-2
EUR 542.4

pCR4-TOPO-RNF135 Plasmid

PVTB01189-2 2 ug
EUR 427.2

pGEM-Ltf(Q25R) Plasmid

PVTB50084-2 2 ug
EUR 427.2

Anti-6xHIS Tag Antibody | CHIS-45A-Z

CHIS-45A-Z 0.1 mg
EUR 348
Description:

Anti-6xHIS Tag Antibody | CHIS-45A-Z | Immunology Consultants Laboratory

Host: Chicken

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | CHIS-45F-Z

CHIS-45F-Z 0.1 mg
EUR 363
Description:

Anti-6xHIS Tag Antibody | CHIS-45F-Z | Immunology Consultants Laboratory

Host: Chicken

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | CHIS-45P-Z

CHIS-45P-Z 0.1 mg
EUR 379
Description:

Anti-6xHIS Tag Antibody | CHIS-45P-Z | Immunology Consultants Laboratory

Host: Chicken

Format: HRP Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | MHIS-45A-Z

MHIS-45A-Z 0.1 mg
EUR 316
Description:

Anti-6xHIS Tag Antibody | MHIS-45A-Z | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-6xHIS Tag Antibody | RHIS-45A-Z

RHIS-45A-Z 0.1 mg
EUR 348
Description:

Anti-6xHIS Tag Antibody | RHIS-45A-Z | Immunology Consultants Laboratory

Host: Rabbit

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | RHIS-45B-Z

RHIS-45B-Z 0.1 mg
EUR 363
Description:

Anti-6xHIS Tag Antibody | RHIS-45B-Z | Immunology Consultants Laboratory

Host: Rabbit

Format: Biotin Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | RHIS-45F-Z

RHIS-45F-Z 0.1 mg
EUR 363
Description:

Anti-6xHIS Tag Antibody | RHIS-45F-Z | Immunology Consultants Laboratory

Host: Rabbit

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Anti-6xHIS Tag Antibody | RHIS-45P-Z

RHIS-45P-Z 0.1 mg
EUR 379
Description:

Anti-6xHIS Tag Antibody | RHIS-45P-Z | Immunology Consultants Laboratory

Host: Rabbit

Format: HRP Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

Multi Fusion-Tagged recombinant Protein 52-Kda containing 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, Flag, S-tag, HA, KT3, E2, Au1, Au5, 6xHis-tags) for ELISA/Western

MFPM52-C 100 ul
EUR 343.2

ToxOut? Endofree Plasmid Midi Kit

K1328-2
EUR 248.4

ToxOut? Endofree Plasmid Maxi Kit

K1330-2
EUR 280.8

ToxOut? Endofree Plasmid Mega3 Kit

K1332-2
EUR 379.2

ToxOut? Endofree Plasmid Mega6 Kit

K1333-2
EUR 444

ToxOut? Endofree Plasmid Mega10 Kit

K1334-2
EUR 510

pMD19-T-LEO1(E225G) Plasmid

PVTB01141-2 2 ug
EUR 427.2

Nori® Human VEGF-C ELISA Kit (2 plates)

GR106472-2 2 x 96-well
EUR 998.4

Nori® Bovine PKA C ELISA Kit- 2 Plates

GR112034-2 2 x 96-well
EUR 998.4

Nori® Porcine PKA C ELISA Kit- 2 Plates

GR113039-2 2 x 96-well
EUR 998.4

Nori® Chicken PKA-C ELISA Kit- 2 Plates

GR114014-2 2 x 96-well
EUR 998.4

Recombinant TMPRSS2 Protein, Partial

E80017
  • EUR 423.50
  • EUR 660.00
  • EUR 2321.00
  • 20 ul
  • 100 ul
  • 1 ml

Recombinant Novel Coronavirus Spike Glycoprotein(S), Partial

E80018
  • EUR 388.30
  • EUR 860.20
  • EUR 4888.40
  • 20 ul
  • 100 ul
  • 1 ml

Express Plasmid Maxiprep Kit (25 min)

K1324-2
EUR 339.6

ToxOut? Endofree ezFilter Plasmid Midi Kit

K1329-2
EUR 248.4

ToxOut? Endofree ezfilter Plasmid Maxi Kit

K1331-2
EUR 280.8

ToxOut? Express Endofree Plasmid Midi Kit

K1335-2
EUR 280.8

ToxOut? Express Endofree Plasmid Maxi Kit

K1336-2
EUR 314.4

Plasmid ezFilter Midi Kit I, Centrifuge

K1316-2
EUR 314.4

Plasmid ezFilter Midi Kit II, Centrifuge

K1317-2
EUR 314.4

Express Plasmid Midiprep Kit (25 min)

K1323-2
EUR 314.4

pCDH-EF1-CymR-T2A-Puro (plasmid)

QM200PA-2 10 ug
EUR 716.4

pCDH-EF1-CymR-T2A-Neo (plasmid)

QM400PA-2 10 ug
EUR 697.2

Nori® Equine PKA C-alpha ELISA Kit- 2 Plates

GR106566-2 2 x 96-well
EUR 998.4

Nori® Human PKA C-alpha ELISA Kit- 2 Plates

GR111082-2 2 x 96-well
EUR 998.4

Nori® Bovine PKA C-alpha ELISA Kit- 2 Plates

GR112035-2 2 x 96-well
EUR 998.4

Nori® Porcine PKA C-alpha ELISA Kit- 2 Plates

GR113068-2 2 x 96-well
EUR 998.4

Nori® Chicken PKA C-alpha ELISA Kit- 2 Plates

GR114015-2 2 x 96-well
EUR 998.4

Monoclonal Anti-Poly-His (6xhis) (His-tag) IgG

HISP14-M 100 ug
EUR 578.4

Phospholipid transfer Antibody

20-abx110096
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Thermal Transfer Ribbon

HIS3233 EACH
EUR 139.08

Propane Transfer System

M-CEM-37015 1 UNIT
EUR 625
Description: Propane Transfer System

Nori® Human Angiopoietin-2 (ANG-2) ELISA Kit (2 plates)

GR106245-2 2 x 96-well
EUR 998.4

Nori Equine MMP-2/TIMP-2 Complex ELISA Kit (2 plates)

GR106502-2 2 x 96-well
EUR 998.4

2-Propyl-β-D-glucuronide, 2 mg

0904-2 2 mg
EUR 194.4

Nori® Human MMP-2/TIMP-2 Complex ELISA Kit (2 plates)

GR106372-2 2 x 96-well
EUR 998.4

Nori® Human b-defensin 2 (BD-2) ELISA Kit- 2 Plates

GR111099-2 2 x 96-well
EUR 998.4

Melanoma; Clone HMB45 (Concentrate)

A00019-C.2 0.2 ml
EUR 283.2

HER-2 / c-erbB-2 / neu / CD340; Clone HRB2/451 (Concentrate)

RA0108-C.1 0.1 ml
EUR 150

HER-2 / c-erbB-2 / neu / CD340; Clone HRB2/451 (Concentrate)

RA0108-C.5 0.5 ml
EUR 360

Monoclonal Anti-Poly-His (6xhis) (His-tag) antibody, ascites

HISP11-M 100 ul
EUR 578.4

Nori Equine MMP-2 ELISA Kit-2 Plates

GR106478-2 2 x 96-well
EUR 998.4

Nori Equine TIM-2 ELISA Kit (2 plates)

GR106516-2 2 x 96-well
EUR 998.4

Nori Human TIM-2 ELISA Kit-2 Plates

GR111039-2 2 x 96-well
EUR 998.4

Nori Canine TIM-2 ELISA Kit (2 plates)

GR115041-2 2 x 96-well
EUR 998.4

2 M NaCl

GR103063-2 200 mL
EUR 70.8

Nori® Chicken Netrin-2 ELISA Kit (2 plates)

GR106184-2 2 x 96-well
EUR 998.4

Nori® Human Tie-2 ELISA Kit (2 plates)

GR106458-2 2 x 96-well
EUR 998.4
Moreover, it interacts with the hydrophilic and hydrophobic components of lipid membranes in addition to with albumin and plasmid DNA. It was noticed that BCE is extra cytotoxic towards colon most cancers cell traces than regular IPEC-J2 cells; it additionally induces apoptosis in most cancers cell traces, however doesn’t induce cell loss of life in regular cells.

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