Transfection With Plasmid Causing Stable Expression of a Foreign Gene Affects General Proteome Pattern in Giardia lamblia Trophozoites

Giardia lamblia is a crucial causative agent of persistent diarrhea in people, home animals, and cattle. Primary analysis is often carried out with the pressure WBC6 and contains genetic manipulations corresponding to transfections. Right here, we examine how transfection with a plasmid inflicting steady expression of a international gene impacts the entire proteome sample. Utilizing shotgun mass spectrometry, we examine the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA).

Moreover GusA, which is detected within the transfected trophozoites solely, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. Particularly, transfection induces antigenic variation. Since transfection inflicting steady expression impacts the proteome sample, transfection experiments ought to bear in mind this impact. As a result of a novel peptide panel, GusA is an instance for an appropriate inside customary for experiments involving transfected cells. Information can be found through ProteomeXchange with identifier PXD022565.

A Klebsiella variicola Plasmid Confers Hypermucoviscosity-Like Phenotype and Alters Capsule Manufacturing and Virulence

Hypermucoviscosity (hmv) is a capsule-associated phenotype often linked with hypervirulent Klebsiella pneumoniae strains. The important thing parts of this phenotype are the RmpADC proteins contained in non-transmissible plasmids recognized and studied in Okay. pneumoniae. Klebsiella variicola is carefully associated to Okay. pneumoniae and lately has been recognized as an emergent human pathogen.

Okay. variicola usually accommodates plasmids, a few of them carrying antibiotic resistance and virulence genes. Beforehand, we described a Okay. variicola scientific isolate displaying an hmv-like phenotype that harbors a 343-kb pKV8917 plasmid. Right here, we investigated whether or not pKV8917 plasmid carried by Okay. variicola 8917 is linked with the hmv-like phenotype and its contribution to virulence. We discovered that curing the 343-kb pKV8917 plasmid induced the lack of hmv, a discount in capsular polysaccharide (P < 0.001) and virulence. As well as, pKV8917 was efficiently transferred to Escherichia coli and Okay. variicola strains through conjugation.

Notably, when pKV8917 was transferred to Okay. variicola, the transconjugants displayed an hmv-like phenotype, and capsule manufacturing and virulence elevated; these phenotypes weren’t noticed within the E. coli transconjugants. These information counsel that the pKV8917 plasmid carries novel hmv and capsule determinants. Complete-plasmid sequencing and evaluation revealed that pKV8917 doesn’t include rmpADC/rmpA2 genes; thus, an alternate mechanism was searched. The 343-kb plasmid accommodates an IncFIB spine and shares a area of ∼150 kb with a 99% identification and 49% protection with a virulence plasmid from hypervirulent Okay. variicola and multidrug-resistant Okay. pneumoniae.

The pKV8917-unique area harbors a cellulose biosynthesis cluster (bcs), fructose- and sucrose-specific (fru/scr) phosphotransferase methods, and the transcriptional regulators araC and iclR, respectively, concerned in membrane permeability. The hmv-like phenotype has been recognized extra ceaselessly, and up to date proof helps the existence of rmpADC/rmpA2-independent hmv-like pathways on this bacterial genus.

Chlamydia trachomatis Plasmid Protein pORF5 Up-Regulates ZFAS1 to Promote Host Cell Survival through MAPK/p38 Pathway

Lengthy non-coding RNAs (lncRNAs) have been demonstrated to play important roles in lots of ailments. Nevertheless, few research have proven that lncRNAs participate within the pathogenesis of Chlamydia trachomatis (C. trachomatis). Right here, we used a lncRNA microarray to detect the worldwide lncRNA expression profiles in HeLa cells transfected with pORF5 plasmid protein, an essential virulence issue for C. trachomatis. The differentially expressed lncRNAs and mRNAs screened by microarray had been chosen for validation by quantitative real-time PCR.

The up-regulated lncRNA zinc finger antisense 1 (ZFAS1) was presumed to concerned in MAPK pathways by bioinformatics evaluation. Inhibition of ZFAS1 decreased the apoptotic fee of pORF5 and decreased the infectivity of C. trachomatis, and MAPK/p38 pathway was concerned in anti-apoptotic impact induced by ZFAS1. Subsequently, the current examine confirmed that pORF5 up-regulates ZFAS1 to advertise host cell survival through MAPK/p38 pathway and influences the infectivity of C. trachomatis.

Progenitor cell-derived exosomes endowed with VEGF plasmids improve osteogenic induction and vascular transforming in massive segmental bone defects

Massive segmental bone regeneration stays an incredible problem as a result of lack of vascularization in newly fashioned bone. Typical methods primarily mix bone scaffolds with seed cells and development elements to modulate osteogenesis and angiogenesis. However, cell-based therapies have some intrinsic points concerning immunogenicity, tumorigenesis, bioactivity and off-the-shelf transplantation. Exosomes are nano-sized (50-200 nm) extracellular vesicles with a fancy composition of proteins, nucleic acids and lipids, that are enticing as therapeutic nanoparticles for illness therapy.

Exosomes even have large potential as fascinating drug/gene supply vectors within the area of regenerative drugs as a consequence of their wonderful biocompatibility and environment friendly mobile internalization.

Strategies: We developed a cell-free tissue engineering system utilizing purposeful exosomes instead of seed cells. Gene-activated engineered exosomes had been constructed by utilizing ATDC5-derived exosomes to encapsulate the VEGF gene. The precise exosomal anchor peptide CP05 acted as a versatile linker and successfully mixed the engineered exosome nanoparticles with 3D-printed porous bone scaffolds.

Outcomes: Our findings demonstrated that engineered exosomes play twin roles as an osteogenic matrix to induce the osteogenic differentiation of mesenchymal stem cells and as a gene vector to controllably launch the VEGF gene to rework the vascular system. In vivo analysis additional verified that the engineered exosome-mediated bone scaffolds may successfully induce the majority of vascularized bone regeneration.

Plasmid Miniprep Kit I
K1312-250	Biovision		EUR 370

Plasmid Miniprep Kit I
K1312-50	Biovision		EUR 283

Plasmid Miniprep Kit II
K1313-250	Biovision		EUR 370

Plasmid Miniprep Kit II
K1313-50	Biovision		EUR 283

Pure Plasmid Miniprep Kit
W0500-200	101Bio	NULL	EUR 0

Pure Plasmid Miniprep Kit
W0500-50	101Bio	NULL	EUR 0

Zenoquick Plasmid Miniprep
Z1004-050	GenDepot	50 preps	EUR 153

Zenoquick Plasmid Miniprep
Z1004-200	GenDepot	200 preps	EUR 317

ApexPrep DNA Plasmid Miniprep Kit
A5001-300	ApexBio	300 tests	EUR 282

ApexPrep DNA Plasmid Miniprep Kit
A5001-50	ApexBio	50 tests	EUR 96

Pure Plasmid 96 Miniprep Kit
W0506-496	101Bio	NULL	EUR 0

Endotoxin-Free Plasmid Miniprep Kit
W2106-50	101Bio	NULL	EUR 0

Plasmid Miniprep Kit (100 preps)
9K-006-0009	Bio Basic	100preps	EUR 205.73
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Plasmid Miniprep Kit (200 preps)
9K-006-0010	Bio Basic	200preps, 200prep	EUR 273.59
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Zenoquick Yeast Plasmid Miniprep
Z1007-100	GenDepot	2x50 preps	EUR 232

LumiPure Plasmid spin miniprep Kit, 50
21583	lumiprobe	50 preps	EUR 78

96-Well Plate Plasmid DNA Miniprep Kit
BS415	Bio Basic	5XPlates, 480prep	EUR 250.31
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96-Well Plate Plasmid DNA Miniprep Kit
BS4152	Bio Basic	2XPlates, 192prep	EUR 155.16
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

ApexPrep DNA Plasmid Miniprep Column Only
A5002-300	ApexBio	300 tests	EUR 247

ApexPrep DNA Plasmid Miniprep Column Only
A5002-50	ApexBio	50 tests	EUR 87

Miniprep Kit For Plasmid DNA Extraction And Purification
MB1005-100Reactions	BosterBio	100 Reactions	EUR 143

Miniprep Kit For Plasmid DNA Extraction And Purification
MB1005-250Reactions	BosterBio	250 Reactions	EUR 259

Miniprep Kit For Plasmid DNA Extraction And Purification
MB1005-50Reactions	BosterBio	50 Reactions	EUR 120

EZ-10 Spin Column Plasmid DNA Miniprep Kit
BS413	Bio Basic	50Preps	EUR 76.64
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

EZ-10 Spin Column Plasmid DNA Miniprep Kit
BS414	Bio Basic	100preps	EUR 95.68
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

ViraBind Adenovirus Miniprep Kit
VPK-099	Cell Biolabs	10 preps	EUR 612
Description: Purification of viruses via cesium chloride ultracentrifugation procedures can be tedious and time-consuming. ViraBind Adenovirus Purification Kits provide a much more efficient system for fast adenoviral purification with high yields. The ViraBind Adenovirus Miniprep Kit uses a special spin column to purify supernatant from a single T75 flask or 10cm dish per prep in about 30 minutes.

Zenoquick RNA Miniprep Kit
Z5005-050	GenDepot	50 preps	EUR 286

Zenoquick RNA Miniprep Kit
Z5005-200	GenDepot	4x50 preps	EUR 847

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid
BS4139	Bio Basic	50Preps	EUR 88.06
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid
BS4149	Bio Basic	100preps	EUR 113.95
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS467	Bio Basic	10Preps	EUR 65.23
Product category: Molecular Biology Kits/DNA - Extraction/Yeast

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS468	Bio Basic	50Preps	EUR 110.9
Product category: Molecular Biology Kits/DNA - Extraction/Yeast

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS469	Bio Basic	250preps	EUR 303.6
Product category: Molecular Biology Kits/DNA - Extraction/Yeast

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92028	Bio Basic	2XPlates, 192prep	EUR 212.25
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92029	Bio Basic	5XPlates, 480prep	EUR 364.5
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Zenoquick Plant RNA Miniprep Kit
Z5016-050	GenDepot	50 prep	EUR 286

SanPrep Column microRNA Miniprep Kit
SK8811	Bio Basic	50prep	EUR 126.1
Product category: Molecular Biology Kits/RNA - microRNA Extraction/Multipurpose

EZ-10 Spin Column Endotoxin-Free Plasmid DNA Miniprep Kit
BS71918	Bio Basic	50Preps	EUR 124.6
Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Prolytic His-Tagged Protein Miniprep Kit
P6400-010	GenDepot	10 prep	EUR 253

Prolytic His-Tagged Protein Miniprep Kit
P6400-050	GenDepot	50 Prep	EUR 658

EZ-10 DNAaway RNA Miniprep Kit
BS88133	Bio Basic	50Preps	EUR 129.93
Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 DNAaway RNA Miniprep Kit
BS88136	Bio Basic	250preps	EUR 379.73
Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT	SBI	1 kit (10 rxn)	EUR 1110
Category: Cas9

96-Well Plate DNA Cleanup Miniprep Kit
BS369	Bio Basic	5XPlates, 480prep	EUR 250.31
Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate DNA Cleanup Miniprep Kit
BS3692	Bio Basic	2xPlates, 192prep	EUR 155.16
Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

One-4-All Genomic DNA Miniprep Kit
BS88503	Bio Basic	50Preps	EUR 116.99
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

One-4-All Genomic DNA Miniprep Kit
BS88504	Bio Basic	100preps	EUR 170.58
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

One-4-All Genomic DNA Miniprep Kit
BS88505	Bio Basic	250preps	EUR 327.96
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

96-Well Plate Viral DNA Miniprep Kit
VT92032	Bio Basic	2XPlates, 192prep	EUR 413.22
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT	SBI	1 Kit	EUR 805
Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT	SBI	10 rxn	EUR 1132
Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
CAS9LIG-KIT	SBI	1 Kit	EUR 153
Category: Cas9

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT	SBI	1 Kit	EUR 4941
Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN340iPS-KIT	SBI	1 Kit	EUR 4941
Category: PinPoint Integrase Tools

EZ-10 Spin Column Total RNA Miniprep Kit
BS1361(SK8655)	Bio Basic	50Preps	EUR 94.15
Product category: Molecular Biology Kits/RNA - Extraction/Animal

EZ-10 Spin Column DNA Cleanup Miniprep Kit
BS367	Bio Basic	50Preps	EUR 76.64
Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

EZ-10 Spin Column DNA Cleanup Miniprep Kit
BS368	Bio Basic	100preps	EUR 95.68
Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437	Bio Basic	5XPlates, 480prep	EUR 362.98
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372	Bio Basic	2XPlates, 192prep	EUR 195.5
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

EZ-10 Spin Column DNA Cleanup Miniprep Kit
BS668	Bio Basic	250preps	EUR 166.58
Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

EZ-10 Spin Column Blood RNA Miniprep Kit
BS82313	Bio Basic	50Preps	EUR 107.09
Product category: Molecular Biology Kits/RNA - Extraction/Blood

EZ-10 Spin Column Plant RNA Miniprep Kit
BS82314	Bio Basic	50Preps	EUR 107.09
Product category: Molecular Biology Kits/RNA - Extraction/Plant

96 Well Plate Yeast Genomic DNA Miniprep Kit
BS8357	Bio Basic	2XPlates, 192prep	EUR 195.5
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

96-Well Plate Plant Genomic DNA Miniprep Kit
BS8361	Bio Basic	2XPlates, 192prep	EUR 195.5
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

EZ-10 Spin Column Fungal RNA Miniprep Kit
BS91915	Bio Basic	50Preps	EUR 107.09
Product category: Molecular Biology Kits/RNA - Extraction/Fungi

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031	Bio Basic	2Plates, 192prep	EUR 175.71
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032	Bio Basic	5Plates, 480prep	EUR 324.92
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292	Bio Basic	2XPlates, 192prep	EUR 175.71
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295	Bio Basic	5XPlates, 480prep	EUR 324.92
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

EZ-10 Spin Column Soil DNA Miniprep Kit
ST82316	Bio Basic	50Preps	EUR 81.97
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

EZ-10 Spin Column Viral DNA Miniprep Kit
VT81812	Bio Basic	50Preps	EUR 224
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

EZ-10 Spin Column Viral DNA Miniprep Kit
VT81813	Bio Basic	250preps	EUR 843
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE620A-KIT	SBI	1 kit	EUR 2132
Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE622A-KIT	SBI	1 kit	EUR 2132
Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE624A-KIT	SBI	1 kit	EUR 2132
Category: Gene Editing

vWF Acty. Kit
ABP-ACT-KIT	Abpcorp	12 x 8 microwells	EUR 428

vWF Ant. Kit
ABP-TOT-KIT	Abpcorp	12 x 8 microwells	EUR 394

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS423	Bio Basic	50Preps	EUR 81.97
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

EZ-10 Spin Column Animal Genomic DNA Miniprep Kit
BS427	Bio Basic	50Preps	EUR 81.97
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

EZ-10 Spin Column Total RNA Miniprep Super Kit
BS583	Bio Basic	20Preps	EUR 76.64
Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 Spin Column Total RNA Miniprep Super Kit
BS584	Bio Basic	100preps	EUR 164.19
Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585	Bio Basic	5XPlates, 480prep	EUR 487.82
Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852	Bio Basic	2XPlates, 192prep	EUR 250.31
Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit
BS624	Bio Basic	250preps	EUR 193.22
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

EZ-10 Spin Column Animal Genomic DNA Miniprep Kit
BS628	Bio Basic	250preps	EUR 193.22
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

EZ-10 Spin Column Total RNA Miniprep Super Kit
BS784	Bio Basic	250preps	EUR 288.38
Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 Spin Column Animal Total RNA Miniprep Kit
BS82312	Bio Basic	50Preps	EUR 97.2
Product category: Molecular Biology Kits/RNA - Extraction/Animal

EZ-10 Spin Column Animal Total RNA Miniprep Kit
BS82322	Bio Basic	250preps	EUR 248.79
Product category: Molecular Biology Kits/RNA - Extraction/Animal

EZ-10 Spin Column Yeast Genomic DNA Miniprep Kit
BS8257	Bio Basic	50Preps	EUR 94.15
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

EZ-10 Total RNA Miniprep Kit (with Proteinase K)
BS88583	Bio Basic	50Preps	EUR 107.09
Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 Spin Column Fungal Genomic DNA Miniprep Kit
FT82012	Bio Basic	50Preps	EUR 81.97
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Fungi

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit
SK8253	Bio Basic	50Preps	EUR 83.4
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit
SK8254	Bio Basic	100preps	EUR 120.72
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

EZ-10 Spin Column Plant Genomic DNA Miniprep Kit
SK8262	Bio Basic	100preps	EUR 120.72
Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)
CAS620A-KIT	SBI	1 kit	EUR 2152
Category: Cas9
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN300A-KIT	SBI	1 Kit	EUR 2798
Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN400A-KIT	SBI	1 Kit	EUR 2798
Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN410A-KIT	SBI	1 Kit	EUR 4335
Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN412A-KIT	SBI	1 Kit	EUR 4335
Category: PinPoint Integrase Tools

PrecisionX Multiplex gRNA Cloning Kit
CAS9-GRNA-KIT	SBI	10 rxn	EUR 445
Category: Cas9

mRNAExpress mRNA Synthesis kit (5 reactions)
MR-KIT-1	SBI	5 reactions	EUR 1152
Category: Stem Cell Products

All-In-One DNA/RNA Miniprep Kit (Cell, Tissue, Plant)
BS88203	Bio Basic	50Preps	EUR 122.32
Product category: Molecular Biology Kits/DNA/RNA/Protein - Extraction/Multipurpose

All-In-One DNA/RNA/Protein Miniprep Kit (Cell, Tissue, Plant)
BS88003	Bio Basic	50Preps	EUR 129.93
Product category: Molecular Biology Kits/DNA/RNA/Protein - Extraction/Multipurpose

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody
EXOAB-KIT-1	SBI	25 ul each	EUR 627
Category: Exosomes

CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)
CASCL9-100A-KIT	SBI	1 Kit	EUR 1132
Category: Cas9

AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS
AP-STR-KIT-P	CORNING	1/pk	EUR 721
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET STARTER KIT 1 AP-20, AP-200 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS
AP-STR-KIT-1	CORNING	1/pk	EUR 355
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET STARTER KIT 2 AP-10, AP-100 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS
AP-STR-KIT-2	CORNING	1/pk	EUR 367
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

FastPure Plasmid Mini Kit
DC201	Vazyme	100 rxn	EUR 128

HiPure Plasmid MaxiPrep Kit
abx298028-10rxns	Abbexa	10 rxns	EUR 286
Shipped within 5-10 working days.

Human KIT shRNA Plasmid
20-abx952584	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

Mouse KIT shRNA Plasmid
20-abx971198	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

HiYield Plasmid Mini Kit
FYG001-100P	Yeastern Biotech	100 Preps	Ask for price

HiYield Plasmid Mini Kit
FYG001-300P	Yeastern Biotech	300 Preps	Ask for price

Plasmid Midi Kit I
K1314-10	Biovision		EUR 332

Plasmid Midi Kit I
K1314-2	Biovision		EUR 262

Plasmid Midi Kit I
K1314-25	Biovision		EUR 392

Plasmid Midi Kit II
K1315-10	Biovision		EUR 370

Plasmid Midi Kit II
K1315-2	Biovision		EUR 262

Plasmid Midi Kit II
K1315-25	Biovision		EUR 425

Plasmid ezFilter Maxi Kit
K1319-10	Biovision		EUR 392

Plasmid ezFilter Maxi Kit
K1319-25	Biovision		EUR 610

Plasmid ezFilter Mega3 Kit
K1320-1	Biovision		EUR 262

Plasmid ezFilter Mega3 Kit
K1320-10	Biovision		EUR 887

Plasmid ezFilter Mega3 Kit
K1320-2	Biovision		EUR 343

Plasmid ezFilter Mega6 Kit
K1321-1	Biovision		EUR 283

Plasmid ezFilter Mega6 Kit
K1321-10	Biovision		EUR 968

Plasmid ezFilter Mega6 Kit
K1321-2	Biovision		EUR 370

Plasmid ezFilter Mega10 Kit
K1322-1	Biovision		EUR 343

Plasmid ezFilter Mega10 Kit
K1322-10	Biovision		EUR 1104

Conclusion: In our present work, we designed particularly engineered exosomes based mostly on the necessities of vascularized bone restore in segmental bone defects. This work concurrently illuminates the potential of purposeful exosomes in acellular tissue engineering.