Giardia lamblia is a crucial causative agent of persistent diarrhea in people, home animals, and cattle. Primary analysis is often carried out with the pressure WBC6 and contains genetic manipulations corresponding to transfections. Right here, we examine how transfection with a plasmid inflicting steady expression of a international gene impacts the entire proteome sample. Utilizing shotgun mass spectrometry, we examine the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA).
Moreover GusA, which is detected within the transfected trophozoites solely, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. Particularly, transfection induces antigenic variation. Since transfection inflicting steady expression impacts the proteome sample, transfection experiments ought to bear in mind this impact. As a result of a novel peptide panel, GusA is an instance for an appropriate inside customary for experiments involving transfected cells. Information can be found through ProteomeXchange with identifier PXD022565.
A Klebsiella variicola Plasmid Confers Hypermucoviscosity-Like Phenotype and Alters Capsule Manufacturing and Virulence
Hypermucoviscosity (hmv) is a capsule-associated phenotype often linked with hypervirulent Klebsiella pneumoniae strains. The important thing parts of this phenotype are the RmpADC proteins contained in non-transmissible plasmids recognized and studied in Okay. pneumoniae. Klebsiella variicola is carefully associated to Okay. pneumoniae and lately has been recognized as an emergent human pathogen.
Okay. variicola usually accommodates plasmids, a few of them carrying antibiotic resistance and virulence genes. Beforehand, we described a Okay. variicola scientific isolate displaying an hmv-like phenotype that harbors a 343-kb pKV8917 plasmid. Right here, we investigated whether or not pKV8917 plasmid carried by Okay. variicola 8917 is linked with the hmv-like phenotype and its contribution to virulence. We discovered that curing the 343-kb pKV8917 plasmid induced the lack of hmv, a discount in capsular polysaccharide (P < 0.001) and virulence. As well as, pKV8917 was efficiently transferred to Escherichia coli and Okay. variicola strains through conjugation.
Notably, when pKV8917 was transferred to Okay. variicola, the transconjugants displayed an hmv-like phenotype, and capsule manufacturing and virulence elevated; these phenotypes weren’t noticed within the E. coli transconjugants. These information counsel that the pKV8917 plasmid carries novel hmv and capsule determinants. Complete-plasmid sequencing and evaluation revealed that pKV8917 doesn’t include rmpADC/rmpA2 genes; thus, an alternate mechanism was searched. The 343-kb plasmid accommodates an IncFIB spine and shares a area of ∼150 kb with a 99% identification and 49% protection with a virulence plasmid from hypervirulent Okay. variicola and multidrug-resistant Okay. pneumoniae.
The pKV8917-unique area harbors a cellulose biosynthesis cluster (bcs), fructose- and sucrose-specific (fru/scr) phosphotransferase methods, and the transcriptional regulators araC and iclR, respectively, concerned in membrane permeability. The hmv-like phenotype has been recognized extra ceaselessly, and up to date proof helps the existence of rmpADC/rmpA2-independent hmv-like pathways on this bacterial genus.

Chlamydia trachomatis Plasmid Protein pORF5 Up-Regulates ZFAS1 to Promote Host Cell Survival through MAPK/p38 Pathway
Lengthy non-coding RNAs (lncRNAs) have been demonstrated to play important roles in lots of ailments. Nevertheless, few research have proven that lncRNAs participate within the pathogenesis of Chlamydia trachomatis (C. trachomatis). Right here, we used a lncRNA microarray to detect the worldwide lncRNA expression profiles in HeLa cells transfected with pORF5 plasmid protein, an essential virulence issue for C. trachomatis. The differentially expressed lncRNAs and mRNAs screened by microarray had been chosen for validation by quantitative real-time PCR.
The up-regulated lncRNA zinc finger antisense 1 (ZFAS1) was presumed to concerned in MAPK pathways by bioinformatics evaluation. Inhibition of ZFAS1 decreased the apoptotic fee of pORF5 and decreased the infectivity of C. trachomatis, and MAPK/p38 pathway was concerned in anti-apoptotic impact induced by ZFAS1. Subsequently, the current examine confirmed that pORF5 up-regulates ZFAS1 to advertise host cell survival through MAPK/p38 pathway and influences the infectivity of C. trachomatis.
Progenitor cell-derived exosomes endowed with VEGF plasmids improve osteogenic induction and vascular transforming in massive segmental bone defects
Massive segmental bone regeneration stays an incredible problem as a result of lack of vascularization in newly fashioned bone. Typical methods primarily mix bone scaffolds with seed cells and development elements to modulate osteogenesis and angiogenesis. However, cell-based therapies have some intrinsic points concerning immunogenicity, tumorigenesis, bioactivity and off-the-shelf transplantation. Exosomes are nano-sized (50-200 nm) extracellular vesicles with a fancy composition of proteins, nucleic acids and lipids, that are enticing as therapeutic nanoparticles for illness therapy.
Exosomes even have large potential as fascinating drug/gene supply vectors within the area of regenerative drugs as a consequence of their wonderful biocompatibility and environment friendly mobile internalization.
Strategies: We developed a cell-free tissue engineering system utilizing purposeful exosomes instead of seed cells. Gene-activated engineered exosomes had been constructed by utilizing ATDC5-derived exosomes to encapsulate the VEGF gene. The precise exosomal anchor peptide CP05 acted as a versatile linker and successfully mixed the engineered exosome nanoparticles with 3D-printed porous bone scaffolds.
Outcomes: Our findings demonstrated that engineered exosomes play twin roles as an osteogenic matrix to induce the osteogenic differentiation of mesenchymal stem cells and as a gene vector to controllably launch the VEGF gene to rework the vascular system. In vivo analysis additional verified that the engineered exosome-mediated bone scaffolds may successfully induce the majority of vascularized bone regeneration.
Plasmid Miniprep Kit II |
K1313-50 |
Biovision |
each |
EUR 339.6 |
Zenoquick Plasmid Miniprep |
Z1004-050 |
GenDepot |
50 preps |
EUR 183.6 |
Zenoquick Plasmid Miniprep |
Z1004-200 |
GenDepot |
200 preps |
EUR 380.4 |
ApexPrep DNA Plasmid Miniprep Kit |
A5001-300 |
ApexBio |
300 tests |
EUR 338.4 |
ApexPrep DNA Plasmid Miniprep Kit |
A5001-50 |
ApexBio |
50 tests |
EUR 115.2 |
Plasmid Miniprep Kit (100 preps) |
9K-006-0009 |
Bio Basic |
100preps |
EUR 246.88 |
|
Plasmid Miniprep Kit (200 preps) |
9K-006-0010 |
Bio Basic |
200preps, 200prep |
EUR 328.31 |
|
Zenoquick Yeast Plasmid Miniprep |
Z1007-100 |
GenDepot |
2x50 preps |
EUR 278.4 |
LumiPure Plasmid spin miniprep Kit, 50 |
21583 |
Lumiprobe |
50 preps |
EUR 93.6 |
96-Well Plate Plasmid DNA Miniprep Kit |
BS415 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
96-Well Plate Plasmid DNA Miniprep Kit |
BS4152 |
Bio Basic |
2XPlates, 192prep |
EUR 186.19 |
|
ApexPrep DNA Plasmid Miniprep Column Only |
A5002-300 |
ApexBio |
300 tests |
EUR 296.4 |
ApexPrep DNA Plasmid Miniprep Column Only |
A5002-50 |
ApexBio |
50 tests |
EUR 104.4 |
EZ-10 Spin Column Plasmid DNA Miniprep Kit |
BS413 |
Bio Basic |
50Preps |
EUR 91.97 |
|
EZ-10 Spin Column Plasmid DNA Miniprep Kit |
BS414 |
Bio Basic |
100preps |
EUR 114.82 |
|
Miniprep Kit For Plasmid DNA Extraction And Purification |
MB1005-100Reactions |
BosterBio |
100 Reactions |
EUR 171.6 |
Miniprep Kit For Plasmid DNA Extraction And Purification |
MB1005-250Reactions |
BosterBio |
250 Reactions |
EUR 310.8 |
Miniprep Kit For Plasmid DNA Extraction And Purification |
MB1005-50Reactions |
BosterBio |
50 Reactions |
EUR 144 |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
ViraBind Adenovirus Miniprep Kit |
VPK-099 |
Cell Biolabs |
10 preps |
EUR 734.4 |
Description: Purification of viruses via cesium chloride ultracentrifugation procedures can be tedious and time-consuming. ViraBind Adenovirus Purification Kits provide a much more efficient system for fast adenoviral purification with high yields. The ViraBind Adenovirus Miniprep Kit uses a special spin column to purify supernatant from a single T75 flask or 10cm dish per prep in about 30 minutes. |
Zenoquick RNA Miniprep Kit |
Z5005-050 |
GenDepot |
50 preps |
EUR 343.2 |
Zenoquick RNA Miniprep Kit |
Z5005-200 |
GenDepot |
4x50 preps |
EUR 1016.4 |
EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid |
BS4139 |
Bio Basic |
50Preps |
EUR 105.67 |
|
EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid |
BS4149 |
Bio Basic |
100preps |
EUR 136.74 |
|
EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit |
BS467 |
Bio Basic |
10Preps |
EUR 78.28 |
|
EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit |
BS468 |
Bio Basic |
50Preps |
EUR 133.08 |
|
EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit |
BS469 |
Bio Basic |
250preps |
EUR 364.32 |
|
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
BS92028 |
Bio Basic |
2XPlates, 192prep |
EUR 254.7 |
|
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
BS92029 |
Bio Basic |
5XPlates, 480prep |
EUR 437.4 |
|
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV200PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV205PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV220PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV225PA-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
SanPrep Column microRNA Miniprep Kit |
SK8811 |
Bio Basic |
50prep |
EUR 151.32 |
|
Zenoquick Plant RNA Miniprep Kit |
Z5016-050 |
GenDepot |
50 prep |
EUR 343.2 |
EZ-10 Spin Column Endotoxin-Free Plasmid DNA Miniprep Kit |
BS71918 |
Bio Basic |
50Preps |
EUR 149.52 |
|
EZ-10 DNAaway RNA Miniprep Kit |
BS88133 |
Bio Basic |
50Preps |
EUR 155.92 |
|
EZ-10 DNAaway RNA Miniprep Kit |
BS88136 |
Bio Basic |
250preps |
EUR 455.68 |
|
Prolytic His-Tagged Protein Miniprep Kit |
P6400-010 |
GenDepot |
10 prep |
EUR 303.6 |
Prolytic His-Tagged Protein Miniprep Kit |
P6400-050 |
GenDepot |
50 Prep |
EUR 789.6 |
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 148.8 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
Column Packing Kit |
PACK-KIT |
Next Advance |
1pack |
EUR 1242 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
PCR Mycoplasma Detection Kit |
M034-Kit |
TOKU-E |
Kit |
EUR 319.2 |
96-Well Plate DNA Cleanup Miniprep Kit |
BS369 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
96-Well Plate DNA Cleanup Miniprep Kit |
BS3692 |
Bio Basic |
2xPlates, 192prep |
EUR 186.19 |
|
One-4-All Genomic DNA Miniprep Kit |
BS88503 |
Bio Basic |
50Preps |
EUR 140.39 |
|
One-4-All Genomic DNA Miniprep Kit |
BS88504 |
Bio Basic |
100preps |
EUR 204.7 |
|
One-4-All Genomic DNA Miniprep Kit |
BS88505 |
Bio Basic |
250preps |
EUR 393.55 |
|
96-Well Plate Viral DNA Miniprep Kit |
VT92032 |
Bio Basic |
2XPlates, 192prep |
EUR 495.86 |
|
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1332 |
|
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
CAS410A-KIT |
SBI |
1 kit (10 rxn) |
EUR 798 |
Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit |
CAS420A-KIT |
SBI |
1 kit (10 rxn) |
EUR 828 |
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS700A-KIT |
SBI |
10 rxn |
EUR 1358.4 |
|
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS720A-KIT |
SBI |
10 rxn |
EUR 1358.4 |
|
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS740A-KIT |
SBI |
10 rxn |
EUR 1358.4 |
|
96 Well Plate Blood Genomic DNA Miniprep Kit |
BT92031 |
Bio Basic |
2Plates, 192prep |
EUR 210.85 |
|
96 Well Plate Blood Genomic DNA Miniprep Kit |
BT92032 |
Bio Basic |
5Plates, 480prep |
EUR 389.9 |
|
EZ-10 Spin Column Total RNA Miniprep Kit |
BS1361(SK8655) |
Bio Basic |
50Preps |
EUR 112.98 |
|
EZ-10 Spin Column DNA Cleanup Miniprep Kit |
BS367 |
Bio Basic |
50Preps |
EUR 91.97 |
|
EZ-10 Spin Column DNA Cleanup Miniprep Kit |
BS368 |
Bio Basic |
100preps |
EUR 114.82 |
|
96-Well Plate Animal Genomic DNA Miniprep Kit |
BS437 |
Bio Basic |
5XPlates, 480prep |
EUR 435.58 |
|
96-Well Plate Animal Genomic DNA Miniprep Kit |
BS4372 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
EZ-10 Spin Column DNA Cleanup Miniprep Kit |
BS668 |
Bio Basic |
250preps |
EUR 199.9 |
|
EZ-10 Spin Column Blood RNA Miniprep Kit |
BS82313 |
Bio Basic |
50Preps |
EUR 128.51 |
|
EZ-10 Spin Column Plant RNA Miniprep Kit |
BS82314 |
Bio Basic |
50Preps |
EUR 128.51 |
|
96 Well Plate Yeast Genomic DNA Miniprep Kit |
BS8357 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
96-Well Plate Plant Genomic DNA Miniprep Kit |
BS8361 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
EZ-10 Spin Column Fungal RNA Miniprep Kit |
BS91915 |
Bio Basic |
50Preps |
EUR 128.51 |
|
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
SK1292 |
Bio Basic |
2XPlates, 192prep |
EUR 210.85 |
|
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
SK1295 |
Bio Basic |
5XPlates, 480prep |
EUR 389.9 |
|
EZ-10 Spin Column Soil DNA Miniprep Kit |
ST82316 |
Bio Basic |
50Preps |
EUR 98.36 |
|
EZ-10 Spin Column Viral DNA Miniprep Kit |
VT81812 |
Bio Basic |
50Preps |
EUR 268.8 |
|
EZ-10 Spin Column Viral DNA Miniprep Kit |
VT81813 |
Bio Basic |
250preps |
EUR 1011.6 |
|
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
CAS510A-KIT |
SBI |
1 Kit |
EUR 966 |
|
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS750A-KIT |
SBI |
10 rxn |
EUR 1358.4 |
|
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS770A-KIT |
SBI |
10 rxn |
EUR 1358.4 |
|
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS790A-KIT |
SBI |
10 rxn |
EUR 1358.4 |
|
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
CAS9LIG-KIT |
SBI |
1 Kit |
EUR 183.6 |
|
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN320A-KIT |
SBI |
1 Kit |
EUR 5929.2 |
|
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN340iPS-KIT |
SBI |
1 Kit |
EUR 5929.2 |
|
EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit |
BS423 |
Bio Basic |
50Preps |
EUR 98.36 |
|
EZ-10 Spin Column Animal Genomic DNA Miniprep Kit |
BS427 |
Bio Basic |
50Preps |
EUR 98.36 |
|
EZ-10 Spin Column Total RNA Miniprep Super Kit |
BS583 |
Bio Basic |
20Preps |
EUR 91.97 |
|
EZ-10 Spin Column Total RNA Miniprep Super Kit |
BS584 |
Bio Basic |
100preps |
EUR 197.03 |
|
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
BS585 |
Bio Basic |
5XPlates, 480prep |
EUR 585.38 |
|
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
BS5852 |
Bio Basic |
2XPlates, 192prep |
EUR 300.37 |
|
EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit |
BS624 |
Bio Basic |
250preps |
EUR 231.86 |
|
EZ-10 Spin Column Animal Genomic DNA Miniprep Kit |
BS628 |
Bio Basic |
250preps |
EUR 231.86 |
|
EZ-10 Spin Column Total RNA Miniprep Super Kit |
BS784 |
Bio Basic |
250preps |
EUR 346.06 |
|
EZ-10 Spin Column Animal Total RNA Miniprep Kit |
BS82312 |
Bio Basic |
50Preps |
EUR 116.64 |
|
EZ-10 Spin Column Animal Total RNA Miniprep Kit |
BS82322 |
Bio Basic |
250preps |
EUR 298.55 |
|
EZ-10 Spin Column Yeast Genomic DNA Miniprep Kit |
BS8257 |
Bio Basic |
50Preps |
EUR 112.98 |
|
EZ-10 Total RNA Miniprep Kit (with Proteinase K) |
BS88583 |
Bio Basic |
50Preps |
EUR 128.51 |
|
EZ-10 Spin Column Fungal Genomic DNA Miniprep Kit |
FT82012 |
Bio Basic |
50Preps |
EUR 98.36 |
|
EZ-10 Spin Column Blood Genomic DNA Miniprep Kit |
SK8253 |
Bio Basic |
50Preps |
EUR 100.08 |
|
EZ-10 Spin Column Blood Genomic DNA Miniprep Kit |
SK8254 |
Bio Basic |
100preps |
EUR 144.86 |
|
EZ-10 Spin Column Plant Genomic DNA Miniprep Kit |
SK8262 |
Bio Basic |
100preps |
EUR 144.86 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE620A-KIT |
SBI |
1 kit |
EUR 2558.4 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE622A-KIT |
SBI |
1 kit |
EUR 2558.4 |
|
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
GE624A-KIT |
SBI |
1 kit |
EUR 2558.4 |
|
vWF Acty. Kit |
ABP-ACT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 513.6 |
vWF Ant. Kit |
ABP-TOT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 472.8 |
All-In-One DNA/RNA Miniprep Kit (Cell, Tissue, Plant) |
BS88203 |
Bio Basic |
50Preps |
EUR 146.78 |
|
PrecisionX Multiplex gRNA Cloning Kit |
CAS9-GRNA-KIT |
SBI |
10 rxn |
EUR 534 |
|
hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9) |
CAS620A-KIT |
SBI |
1 kit |
EUR 2582.4 |
|
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression) |
PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN300A-KIT |
SBI |
1 Kit |
EUR 3357.6 |
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN400A-KIT |
SBI |
1 Kit |
EUR 3357.6 |
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN410A-KIT |
SBI |
1 Kit |
EUR 5202 |
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN412A-KIT |
SBI |
1 Kit |
EUR 5202 |
|
mRNAExpress mRNA Synthesis kit (5 reactions) |
MR-KIT-1 |
SBI |
5 reactions |
EUR 1382.4 |
|
AxioCam Erc5s rev 2, w/Accessory Kit |
ZV-Erc5sAC-Kit |
MiTeGen |
1 KIT |
EUR 1611 |
|
Description: AxioCam Erc5s rev 2, w/Accessory Kit- connection cable Y-USB 2.0.- HDMI cable to DVI-D- SD card- IR remote control |
All-In-One DNA/RNA/Protein Miniprep Kit (Cell, Tissue, Plant) |
BS88003 |
Bio Basic |
50Preps |
EUR 155.92 |
|
ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody |
EXOAB-KIT-1 |
SBI |
25 ul each |
EUR 752.4 |
|
CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent) |
CASCL9-100A-KIT |
SBI |
1 Kit |
EUR 1358.4 |
|
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-520A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-550A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-575A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-599A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-639A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-649A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-659A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-669A-KIT |
SBI |
1 kit |
EUR 1162 |
pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-619A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-629A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-589A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-609A-KIT |
SBI |
1 kit |
EUR 1141 |
AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS |
AP-STR-KIT-P |
CORNING |
1/pk |
EUR 865.2 |
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
AXYPET STARTER KIT 1 AP-20, AP-200 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS |
AP-STR-KIT-1 |
CORNING |
1/pk |
EUR 426 |
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
AXYPET STARTER KIT 2 AP-10, AP-100 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS |
AP-STR-KIT-2 |
CORNING |
1/pk |
EUR 440.4 |
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
HiPure Plasmid MaxiPrep Kit |
abx298028-10rxns |
Abbexa |
10 rxns |
EUR 343.2 |
|
FastPure Plasmid Mini Kit |
DC201 |
Vazyme |
100 rxn |
EUR 153.6 |
Human KIT shRNA Plasmid |
20-abx952584 |
Abbexa |
|
|
|
Mouse KIT shRNA Plasmid |
20-abx971198 |
Abbexa |
|
|
|
Conclusion: In our present work, we designed particularly engineered exosomes based mostly on the necessities of vascularized bone restore in segmental bone defects. This work concurrently illuminates the potential of purposeful exosomes in acellular tissue engineering.