Transfection With Plasmid Causing Stable Expression of a Foreign Gene Affects General Proteome Pattern in Giardia lamblia Trophozoites

Giardia lamblia is a crucial causative agent of persistent diarrhea in people, home animals, and cattle. Primary analysis is often carried out with the pressure WBC6 and contains genetic manipulations corresponding to transfections. Right here, we examine how transfection with a plasmid inflicting steady expression of a international gene impacts the entire proteome sample. Utilizing shotgun mass spectrometry, we examine the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA).

Moreover GusA, which is detected within the transfected trophozoites solely, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. Particularly, transfection induces antigenic variation. Since transfection inflicting steady expression impacts the proteome sample, transfection experiments ought to bear in mind this impact. As a result of a novel peptide panel, GusA is an instance for an appropriate inside customary for experiments involving transfected cells. Information can be found through ProteomeXchange with identifier PXD022565.

Klebsiella variicola Plasmid Confers Hypermucoviscosity-Like Phenotype and Alters Capsule Manufacturing and Virulence

Hypermucoviscosity (hmv) is a capsule-associated phenotype often linked with hypervirulent Klebsiella pneumoniae strains. The important thing parts of this phenotype are the RmpADC proteins contained in non-transmissible plasmids recognized and studied in Okay. pneumoniaeKlebsiella variicola is carefully associated to Okay. pneumoniae and lately has been recognized as an emergent human pathogen.

Okay. variicola usually accommodates plasmids, a few of them carrying antibiotic resistance and virulence genes. Beforehand, we described a Okay. variicola scientific isolate displaying an hmv-like phenotype that harbors a 343-kb pKV8917 plasmid. Right here, we investigated whether or not pKV8917 plasmid carried by Okay. variicola 8917 is linked with the hmv-like phenotype and its contribution to virulence. We discovered that curing the 343-kb pKV8917 plasmid induced the lack of hmv, a discount in capsular polysaccharide (P < 0.001) and virulence. As well as, pKV8917 was efficiently transferred to Escherichia coli and Okay. variicola strains through conjugation.

Notably, when pKV8917 was transferred to Okay. variicola, the transconjugants displayed an hmv-like phenotype, and capsule manufacturing and virulence elevated; these phenotypes weren’t noticed within the E. coli transconjugants. These information counsel that the pKV8917 plasmid carries novel hmv and capsule determinants. Complete-plasmid sequencing and evaluation revealed that pKV8917 doesn’t include rmpADC/rmpA2 genes; thus, an alternate mechanism was searched. The 343-kb plasmid accommodates an IncFIB spine and shares a area of ∼150 kb with a 99% identification and 49% protection with a virulence plasmid from hypervirulent Okay. variicola and multidrug-resistant Okay. pneumoniae.

The pKV8917-unique area harbors a cellulose biosynthesis cluster (bcs), fructose- and sucrose-specific (fru/scr) phosphotransferase methods, and the transcriptional regulators araC and iclR, respectively, concerned in membrane permeability. The hmv-like phenotype has been recognized extra ceaselessly, and up to date proof helps the existence of rmpADC/rmpA2-independent hmv-like pathways on this bacterial genus.

Transfection With Plasmid Causing Stable Expression of a Foreign Gene Affects General Proteome Pattern in Giardia lamblia Trophozoites

Chlamydia trachomatis Plasmid Protein pORF5 Up-Regulates ZFAS1 to Promote Host Cell Survival through MAPK/p38 Pathway

Lengthy non-coding RNAs (lncRNAs) have been demonstrated to play important roles in lots of ailments. Nevertheless, few research have proven that lncRNAs participate within the pathogenesis of Chlamydia trachomatis (C. trachomatis). Right here, we used a lncRNA microarray to detect the worldwide lncRNA expression profiles in HeLa cells transfected with pORF5 plasmid protein, an essential virulence issue for C. trachomatis. The differentially expressed lncRNAs and mRNAs screened by microarray had been chosen for validation by quantitative real-time PCR.

The up-regulated lncRNA zinc finger antisense 1 (ZFAS1) was presumed to concerned in MAPK pathways by bioinformatics evaluation. Inhibition of ZFAS1 decreased the apoptotic fee of pORF5 and decreased the infectivity of C. trachomatis, and MAPK/p38 pathway was concerned in anti-apoptotic impact induced by ZFAS1. Subsequently, the current examine confirmed that pORF5 up-regulates ZFAS1 to advertise host cell survival through MAPK/p38 pathway and influences the infectivity of C. trachomatis.

Progenitor cell-derived exosomes endowed with VEGF plasmids improve osteogenic induction and vascular transforming in massive segmental bone defects

Massive segmental bone regeneration stays an incredible problem as a result of lack of vascularization in newly fashioned bone. Typical methods primarily mix bone scaffolds with seed cells and development elements to modulate osteogenesis and angiogenesis. However, cell-based therapies have some intrinsic points concerning immunogenicity, tumorigenesis, bioactivity and off-the-shelf transplantation. Exosomes are nano-sized (50-200 nm) extracellular vesicles with a fancy composition of proteins, nucleic acids and lipids, that are enticing as therapeutic nanoparticles for illness therapy.

Exosomes even have large potential as fascinating drug/gene supply vectors within the area of regenerative drugs as a consequence of their wonderful biocompatibility and environment friendly mobile internalization.

Strategies: We developed a cell-free tissue engineering system utilizing purposeful exosomes instead of seed cells. Gene-activated engineered exosomes had been constructed by utilizing ATDC5-derived exosomes to encapsulate the VEGF gene. The precise exosomal anchor peptide CP05 acted as a versatile linker and successfully mixed the engineered exosome nanoparticles with 3D-printed porous bone scaffolds.

Outcomes: Our findings demonstrated that engineered exosomes play twin roles as an osteogenic matrix to induce the osteogenic differentiation of mesenchymal stem cells and as a gene vector to controllably launch the VEGF gene to rework the vascular system. In vivo analysis additional verified that the engineered exosome-mediated bone scaffolds may successfully induce the majority of vascularized bone regeneration.

Plasmid Miniprep Kit I

K1312-250
EUR 370

Plasmid Miniprep Kit I

K1312-50
EUR 283

Plasmid Miniprep Kit II

K1313-250
EUR 370

Plasmid Miniprep Kit II

K1313-50
EUR 283

Pure Plasmid Miniprep Kit

W0500-200 NULL
EUR 0

Pure Plasmid Miniprep Kit

W0500-50 NULL
EUR 0

Zenoquick Plasmid Miniprep

Z1004-050 50 preps
EUR 153

Zenoquick Plasmid Miniprep

Z1004-200 200 preps
EUR 317

ApexPrep DNA Plasmid Miniprep Kit

A5001-300 300 tests
EUR 282

ApexPrep DNA Plasmid Miniprep Kit

A5001-50 50 tests
EUR 96

Pure Plasmid 96 Miniprep Kit

W0506-496 NULL
EUR 0

Endotoxin-Free Plasmid Miniprep Kit

W2106-50 NULL
EUR 0

Plasmid Miniprep Kit (100 preps)

9K-006-0009 100preps
EUR 205.73
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Plasmid Miniprep Kit (200 preps)

9K-006-0010 200preps, 200prep
EUR 273.59
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Zenoquick Yeast Plasmid Miniprep

Z1007-100 2x50 preps
EUR 232

LumiPure Plasmid spin miniprep Kit, 50

21583 50 preps
EUR 78

96-Well Plate Plasmid DNA Miniprep Kit

BS415 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 2XPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

ApexPrep DNA Plasmid Miniprep Column Only

A5002-300 300 tests
EUR 247

ApexPrep DNA Plasmid Miniprep Column Only

A5002-50 50 tests
EUR 87

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-100Reactions 100 Reactions
EUR 143

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-250Reactions 250 Reactions
EUR 259

Miniprep Kit For Plasmid DNA Extraction And Purification

MB1005-50Reactions 50 Reactions
EUR 120

EZ-10 Spin Column Plasmid DNA Miniprep Kit

BS413 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

EZ-10 Spin Column Plasmid DNA Miniprep Kit

BS414 100preps
EUR 95.68
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132
  • Category: Cas9

ViraBind Adenovirus Miniprep Kit

VPK-099 10 preps
EUR 612
Description: Purification of viruses via cesium chloride ultracentrifugation procedures can be tedious and time-consuming. ViraBind Adenovirus Purification Kits provide a much more efficient system for fast adenoviral purification with high yields. The ViraBind Adenovirus Miniprep Kit uses a special spin column to purify supernatant from a single T75 flask or 10cm dish per prep in about 30 minutes.

Zenoquick RNA Miniprep Kit

Z5005-050 50 preps
EUR 286

Zenoquick RNA Miniprep Kit

Z5005-200 4x50 preps
EUR 847

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid

BS4139 50Preps
EUR 88.06
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid

BS4149 100preps
EUR 113.95
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit

BS467 10Preps
EUR 65.23
  • Product category: Molecular Biology Kits/DNA - Extraction/Yeast

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit

BS468 50Preps
EUR 110.9
  • Product category: Molecular Biology Kits/DNA - Extraction/Yeast

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit

BS469 250preps
EUR 303.6
  • Product category: Molecular Biology Kits/DNA - Extraction/Yeast

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92028 2XPlates, 192prep
EUR 212.25
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92029 5XPlates, 480prep
EUR 364.5
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Zenoquick Plant RNA Miniprep Kit

Z5016-050 50 prep
EUR 286

SanPrep Column microRNA Miniprep Kit

SK8811 50prep
EUR 126.1
  • Product category: Molecular Biology Kits/RNA - microRNA Extraction/Multipurpose

EZ-10 Spin Column Endotoxin-Free Plasmid DNA Miniprep Kit

BS71918 50Preps
EUR 124.6
  • Product category: Molecular Biology Kits/DNA - Extraction/Bacteria

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Prolytic His-Tagged Protein Miniprep Kit

P6400-010 10 prep
EUR 253

Prolytic His-Tagged Protein Miniprep Kit

P6400-050 50 Prep
EUR 658

EZ-10 DNAaway RNA Miniprep Kit

BS88133 50Preps
EUR 129.93
  • Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 DNAaway RNA Miniprep Kit

BS88136 250preps
EUR 379.73
  • Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110
  • Category: Cas9

96-Well Plate DNA Cleanup Miniprep Kit

BS369 5XPlates, 480prep
EUR 250.31
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate DNA Cleanup Miniprep Kit

BS3692 2xPlates, 192prep
EUR 155.16
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

One-4-All Genomic DNA Miniprep Kit

BS88503 50Preps
EUR 116.99
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

One-4-All Genomic DNA Miniprep Kit

BS88504 100preps
EUR 170.58
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

One-4-All Genomic DNA Miniprep Kit

BS88505 250preps
EUR 327.96
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

96-Well Plate Viral DNA Miniprep Kit

VT92032 2XPlates, 192prep
EUR 413.22
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132
  • Category: Cas9

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132
  • Category: Cas9

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153
  • Category: Cas9

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941
  • Category: PinPoint Integrase Tools

EZ-10 Spin Column Total RNA Miniprep Kit

BS1361(SK8655) 50Preps
EUR 94.15
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

EZ-10 Spin Column DNA Cleanup Miniprep Kit

BS367 50Preps
EUR 76.64
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

EZ-10 Spin Column DNA Cleanup Miniprep Kit

BS368 100preps
EUR 95.68
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

96-Well Plate Animal Genomic DNA Miniprep Kit

BS437 5XPlates, 480prep
EUR 362.98
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

96-Well Plate Animal Genomic DNA Miniprep Kit

BS4372 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

EZ-10 Spin Column DNA Cleanup Miniprep Kit

BS668 250preps
EUR 166.58
  • Product category: Molecular Biology Kits/DNA - Extraction/Clean-up

EZ-10 Spin Column Blood RNA Miniprep Kit

BS82313 50Preps
EUR 107.09
  • Product category: Molecular Biology Kits/RNA - Extraction/Blood

EZ-10 Spin Column Plant RNA Miniprep Kit

BS82314 50Preps
EUR 107.09
  • Product category: Molecular Biology Kits/RNA - Extraction/Plant

96 Well Plate Yeast Genomic DNA Miniprep Kit

BS8357 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

96-Well Plate Plant Genomic DNA Miniprep Kit

BS8361 2XPlates, 192prep
EUR 195.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

EZ-10 Spin Column Fungal RNA Miniprep Kit

BS91915 50Preps
EUR 107.09
  • Product category: Molecular Biology Kits/RNA - Extraction/Fungi

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 2Plates, 192prep
EUR 175.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 5Plates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1292 2XPlates, 192prep
EUR 175.71
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1295 5XPlates, 480prep
EUR 324.92
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

EZ-10 Spin Column Soil DNA Miniprep Kit

ST82316 50Preps
EUR 81.97
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

EZ-10 Spin Column Viral DNA Miniprep Kit

VT81812 50Preps
EUR 224
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

EZ-10 Spin Column Viral DNA Miniprep Kit

VT81813 250preps
EUR 843
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 428

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 394

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS423 50Preps
EUR 81.97
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

EZ-10 Spin Column Animal Genomic DNA Miniprep Kit

BS427 50Preps
EUR 81.97
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

EZ-10 Spin Column Total RNA Miniprep Super Kit

BS583 20Preps
EUR 76.64
  • Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 Spin Column Total RNA Miniprep Super Kit

BS584 100preps
EUR 164.19
  • Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 5XPlates, 480prep
EUR 487.82
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 2XPlates, 192prep
EUR 250.31
  • Product category: Molecular Biology Kits/RNA - Extraction/Bacteria

EZ-10 Spin Column Bacterial Genomic DNA Miniprep Kit

BS624 250preps
EUR 193.22
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria

EZ-10 Spin Column Animal Genomic DNA Miniprep Kit

BS628 250preps
EUR 193.22
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal

EZ-10 Spin Column Total RNA Miniprep Super Kit

BS784 250preps
EUR 288.38
  • Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 Spin Column Animal Total RNA Miniprep Kit

BS82312 50Preps
EUR 97.2
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

EZ-10 Spin Column Animal Total RNA Miniprep Kit

BS82322 250preps
EUR 248.79
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

EZ-10 Spin Column Yeast Genomic DNA Miniprep Kit

BS8257 50Preps
EUR 94.15
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast

EZ-10 Total RNA Miniprep Kit (with Proteinase K)

BS88583 50Preps
EUR 107.09
  • Product category: Molecular Biology Kits/RNA - Extraction/Multipurpose

EZ-10 Spin Column Fungal Genomic DNA Miniprep Kit

FT82012 50Preps
EUR 81.97
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Fungi

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8253 50Preps
EUR 83.4
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

EZ-10 Spin Column Blood Genomic DNA Miniprep Kit

SK8254 100preps
EUR 120.72
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood

EZ-10 Spin Column Plant Genomic DNA Miniprep Kit

SK8262 100preps
EUR 120.72
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT 1 kit
EUR 2152
  • Category: Cas9
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
EUR 2798
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT 1 Kit
EUR 4335
  • Category: PinPoint Integrase Tools

PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT 10 rxn
EUR 445
  • Category: Cas9

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1152
  • Category: Stem Cell Products

All-In-One DNA/RNA Miniprep Kit (Cell, Tissue, Plant)

BS88203 50Preps
EUR 122.32
  • Product category: Molecular Biology Kits/DNA/RNA/Protein - Extraction/Multipurpose

All-In-One DNA/RNA/Protein Miniprep Kit (Cell, Tissue, Plant)

BS88003 50Preps
EUR 129.93
  • Product category: Molecular Biology Kits/DNA/RNA/Protein - Extraction/Multipurpose

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody

EXOAB-KIT-1 25 ul each
EUR 627
  • Category: Exosomes

CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)

CASCL9-100A-KIT 1 Kit
EUR 1132
  • Category: Cas9

AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS

AP-STR-KIT-P 1/pk
EUR 721
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET STARTER KIT 1 AP-20, AP-200 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS

AP-STR-KIT-1 1/pk
EUR 355
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET STARTER KIT 2 AP-10, AP-100 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS

AP-STR-KIT-2 1/pk
EUR 367
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

FastPure Plasmid Mini Kit

DC201 100 rxn
EUR 128

HiPure Plasmid MaxiPrep Kit

abx298028-10rxns 10 rxns
EUR 286
  • Shipped within 5-10 working days.

Human KIT shRNA Plasmid

20-abx952584
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse KIT shRNA Plasmid

20-abx971198
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

HiYield Plasmid Mini Kit

FYG001-100P 100 Preps Ask for price

HiYield Plasmid Mini Kit

FYG001-300P 300 Preps Ask for price

Plasmid Midi Kit I

K1314-10
EUR 332

Plasmid Midi Kit I

K1314-2
EUR 262

Plasmid Midi Kit I

K1314-25
EUR 392

Plasmid Midi Kit II

K1315-10
EUR 370

Plasmid Midi Kit II

K1315-2
EUR 262

Plasmid Midi Kit II

K1315-25
EUR 425

Plasmid ezFilter Maxi Kit

K1319-10
EUR 392

Plasmid ezFilter Maxi Kit

K1319-25
EUR 610

Plasmid ezFilter Mega3 Kit

K1320-1
EUR 262

Plasmid ezFilter Mega3 Kit

K1320-10
EUR 887

Plasmid ezFilter Mega3 Kit

K1320-2
EUR 343

Plasmid ezFilter Mega6 Kit

K1321-1
EUR 283

Plasmid ezFilter Mega6 Kit

K1321-10
EUR 968

Plasmid ezFilter Mega6 Kit

K1321-2
EUR 370

Plasmid ezFilter Mega10 Kit

K1322-1
EUR 343

Plasmid ezFilter Mega10 Kit

K1322-10
EUR 1104

Conclusion: In our present work, we designed particularly engineered exosomes based mostly on the necessities of vascularized bone restore in segmental bone defects. This work concurrently illuminates the potential of purposeful exosomes in acellular tissue engineering.

 

 

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